Results 31 to 40 of about 399,703 (233)
A Geant4 based engineering tool for Fresnel lenses
, 2007 Geant4 is a Monte Carlo radiation transport toolkit that is becoming a tool of generalized application in areas such as high-energy physics, nuclear physics, astroparticle physics, or medical physics.
Costa, João +2 more
core +1 more source
Anchorage‐independent and faster growth in clonal population from UV‐irradiated NER‐deficient cells
FEBS Open Bio, EarlyView.UV‐irradiated cells expressing a DDB2 mutant protein unable to interact with PCNA (DDB2PCNA‐) form clones able to grow without anchorage. Different experimental approaches reveal heterogeneity in cell cycle regulation and drug response within these clones, emphasizing the crucial role of the DDB2‐PCNA interaction in preventing cellular transformation ...
Paola Perucca +6 more
wiley +1 more source
Computational toolbox for optical tweezers in geometrical optics
, 2014 Optical tweezers have found widespread application in many fields, from physics to biology. Here, we explain in detail how optical forces and torques can be described within the geometrical optics approximation and we show that this approximation ...
Callegari, Agnese +3 more
core +1 more source
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Keldysh Field Theory for Driven Open Quantum Systems
, 2016 Recent experimental developments in diverse areas - ranging from cold atomic gases over light-driven semiconductors to microcavity arrays - move systems into the focus, which are located on the interface of quantum optics, many-body physics and ...
Buchhold, M., Diehl, S., Sieberer, L. M.
core +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Quantum optics and frontiers of physics: The third quantum revolution
, 2016 The year 2015 was the International Year of Light. It marked, however, also the 20th anniversary of the first observation of Bose-Einstein condensation in atomic vapors by Eric Cornell, Carl Wieman and Wolfgang Ketterle.
Ahufinger, Veronica +3 more
core +1 more source
Intercompartmental communication in senescence
FEBS Open Bio, EarlyView.Senescent cells experience structural changes in the plasma membrane, endoplasmic reticulum, mitochondria, lysosomes, nucleus, and cytoskeleton. These alterations disrupt crosstalk among cellular compartments, impairing vesicular trafficking, contact sites, and molecular flow.
Krystyna Mazan‐Mamczarz +3 more
wiley +1 more source
Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis
FEBS Open Bio, EarlyView.Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho +4 more
wiley +1 more source