Results 61 to 70 of about 71,095 (258)
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis
FEBS Open Bio, EarlyView.Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho +4 more
wiley +1 more source
Micro-machined membrane deformable mirrors for cryogenic adaptive optics [PDF]
, 2002 In this Thesis, the suitability of 37 actuator Micro-machined membrane deformable mirrors (MMDM) for adaptive optics at cryogenic temperatures is evaluated. A method is described to safely cool MMDMs to 78K. The initial surface figures of three MMDMs are
Dyson, H.M., Dyson, Harold Michael
core
Subwavelength imaging with materials of in-principle arbitrarily low index contrast [PDF]
, 2012 Perfect imaging with Maxwell's fish eye opens the exciting prospect of passive imaging systems with a resolution no longer limited by the wave nature of light.
Ong, C. K. +4 more
core +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Negative frequency waves in optics : control and investigation of their generation and evolution [PDF]
, 2015 This thesis is concerned with various methods for the control and investigation of pulse dynamics in a Photonic Crystal Fibre (PCF) and of the radiation driven by a short pulse.
McLenaghan, Joanna Siân
core
Cavity Optomechanics with High Stress Silicon Nitride Films [PDF]
, 2012 There has been a barrage of interest in recent years to marry the fields of nanomechanics and quantum optics. Mechanical systems provide sensitive and scalable architectures for sensing applications ranging from atomic force microscopy to gravity wave ...
Wilson, Dalziel Joseph
core +1 more source
FEBS Open Bio, EarlyView.The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley +1 more source
FEBS Open Bio, EarlyView.Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas +6 more
wiley +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source