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Generating Gene Silenced Mutants in Phytophthora sojae
2018Phytophthora species are notorious pathogens of plants and cause enormous damage to agriculture. In order to understand Phytophthora pathogenesis, gene silencing and knockout methods are important for the investigation of gene functions. Although CRISPR/Cas9-based gene knockout procedures have been developed in Phytophthora sojae and Phytophthora ...
Zhao, Yao +4 more
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Journal of Molecular Evolution, 2009
Automated and manual annotation of the ATP binding cassette (ABC) superfamily in the Phytophthora ramorum and P. sojae genomes has identified 135 and 136 members, respectively, indicating that this family is comparable in size to the Arabidopsis thaliana and rice genomes, and significantly larger than that of two fungal pathogens, Fusarium graminearum ...
Paul F, Morris, Vipaporn, Phuntumart
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Automated and manual annotation of the ATP binding cassette (ABC) superfamily in the Phytophthora ramorum and P. sojae genomes has identified 135 and 136 members, respectively, indicating that this family is comparable in size to the Arabidopsis thaliana and rice genomes, and significantly larger than that of two fungal pathogens, Fusarium graminearum ...
Paul F, Morris, Vipaporn, Phuntumart
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Journal of Proteomics, 2015
The recently described oomycete pathogen Phytophthora pisi causes root rot on pea and faba bean, while the closely related Phytophthora sojae is the causal agent of soybean root and stem rot. Differences in the pathogenicity factor repertoires that enable the two species to have distinct host specificity towards pea and soybean, were studied using ...
Hosseini S +10 more
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The recently described oomycete pathogen Phytophthora pisi causes root rot on pea and faba bean, while the closely related Phytophthora sojae is the causal agent of soybean root and stem rot. Differences in the pathogenicity factor repertoires that enable the two species to have distinct host specificity towards pea and soybean, were studied using ...
Hosseini S +10 more
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Pathotypes of Phytophthora sojae and their distribution in Jilin, China
Journal of Plant Pathology, 2021A nine-year survey on pathotypes of Phytophthora sojae from 2007 to 2015 throughout Jilin Province of China was conducted. A total of 219 single-zoospore isolates of P. sojae that came from 250 commercial soybean fields in 25 counties were tested for their virulence phenotypes by using eight soybean differentials representing eight Rps alleles.
Zhuoqun Zhang +5 more
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Identification of Phytophthora sojae genes involved in asexual sporogenesis
Journal of Genetics, 2009To explore the molecular mechanisms involved in asexual spore development in Phytophthora sojae, the zoospores of strain PS26 were treated with ultraviolet (UV) irradiation. After selection, a mutant progeny, termed PS26-U03, was obtained and demonstrated to exhibit no oospore production.
Ziying, Wang +5 more
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Soybean Isoflavones Trigger a Calcium Influx in Phytophthora sojae
Fungal Genetics and Biology, 1999Both the motile zoospores and the hyphal germ tubes of Phytophthora sojae respond chemotropically to the soybean isoflavones daidzein and genistein. The role of Ca(2+) in the cellular response to these host signals was investigated by using X-ray microanalysis of cells to monitor net changes in cellular levels of Ca(2+) and by quantifying the effects ...
M S, Connolly +3 more
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Peroxidase Activity in Soybeans Following Inoculation with Phytophthora sojae
Mycopathologia, 2006The effects of race-specific resistance as conditioned by Rps genes (rps, Rps1-k, Rps2, Rps3, Rps6) in two genetic backgrounds (Williams & Harosoy) on accumulation of soluble peroxidases were determined by a soybean peroxidase capture assay (SPCA) after inoculation with P. sojae races 2, 7, or 25.
Jose C, Melgar +2 more
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Phytophthora sojae effectors orchestrate warfare with host immunity
Current Opinion in Microbiology, 2018Phytophthora sojae is one of the most damaging plant pathogens of soybean. To aid establishment of a compatible interaction with its host, P. sojae deploys many secreted effectors. These effectors act either in the apoplastic space to cope with hostile conditions or inside of host cells to reprogram host physiology favoring pathogen growth.
Yan, Wang, Yuanchao, Wang
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Inheritance and mapping of 11 avirulence genes in Phytophthora sojae
Fungal Genetics and Biology, 2002Two new crosses involving four races (races 7, 16, 17, and 25) of the soybean root and stem rot pathogen Phytophthora sojae were established (7/16 cross; 17/25 cross). An F2 population derived from each cross was used to determine the genetic basis of avirulence towards 11 different resistance genes in soybean.
May, K.J. +7 more
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Glucan synthase of Phytophthora sojae
2002Glucans, with the (1-3)-b-glucosidic linkage as major feature, are present in most of the higher plants, in many lower plants, as well as in microorganisms (Stone and Clarke, 1992). The synthesis of (1-3)-b-glucan in vivo is catalysed by the enzyme (1-3)-b-glucan synthase (EC 2.4.1.34; UDP-glucose:1,3-b-D-glucan 3-b-D-glucosyl transferase) using UDP ...
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