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Generating Gene Silenced Mutants in Phytophthora sojae

2018
Phytophthora species are notorious pathogens of plants and cause enormous damage to agriculture. In order to understand Phytophthora pathogenesis, gene silencing and knockout methods are important for the investigation of gene functions. Although CRISPR/Cas9-based gene knockout procedures have been developed in Phytophthora sojae and Phytophthora ...
Zhao, Yao   +4 more
openaire   +3 more sources

Inventory and Comparative Evolution of the ABC Superfamily in the Genomes of Phytophthora ramorum and Phytophthora sojae

Journal of Molecular Evolution, 2009
Automated and manual annotation of the ATP binding cassette (ABC) superfamily in the Phytophthora ramorum and P. sojae genomes has identified 135 and 136 members, respectively, indicating that this family is comparable in size to the Arabidopsis thaliana and rice genomes, and significantly larger than that of two fungal pathogens, Fusarium graminearum ...
Paul F, Morris, Vipaporn, Phuntumart
openaire   +2 more sources

Comparative proteomic analysis of hyphae and germinating cysts of Phytophthora pisi and Phytophthora sojae

Journal of Proteomics, 2015
The recently described oomycete pathogen Phytophthora pisi causes root rot on pea and faba bean, while the closely related Phytophthora sojae is the causal agent of soybean root and stem rot. Differences in the pathogenicity factor repertoires that enable the two species to have distinct host specificity towards pea and soybean, were studied using ...
Hosseini S   +10 more
openaire   +3 more sources

Pathotypes of Phytophthora sojae and their distribution in Jilin, China

Journal of Plant Pathology, 2021
A nine-year survey on pathotypes of Phytophthora sojae from 2007 to 2015 throughout Jilin Province of China was conducted. A total of 219 single-zoospore isolates of P. sojae that came from 250 commercial soybean fields in 25 counties were tested for their virulence phenotypes by using eight soybean differentials representing eight Rps alleles.
Zhuoqun Zhang   +5 more
openaire   +1 more source

Identification of Phytophthora sojae genes involved in asexual sporogenesis

Journal of Genetics, 2009
To explore the molecular mechanisms involved in asexual spore development in Phytophthora sojae, the zoospores of strain PS26 were treated with ultraviolet (UV) irradiation. After selection, a mutant progeny, termed PS26-U03, was obtained and demonstrated to exhibit no oospore production.
Ziying, Wang   +5 more
openaire   +2 more sources

Soybean Isoflavones Trigger a Calcium Influx in Phytophthora sojae

Fungal Genetics and Biology, 1999
Both the motile zoospores and the hyphal germ tubes of Phytophthora sojae respond chemotropically to the soybean isoflavones daidzein and genistein. The role of Ca(2+) in the cellular response to these host signals was investigated by using X-ray microanalysis of cells to monitor net changes in cellular levels of Ca(2+) and by quantifying the effects ...
M S, Connolly   +3 more
openaire   +2 more sources

Peroxidase Activity in Soybeans Following Inoculation with Phytophthora sojae

Mycopathologia, 2006
The effects of race-specific resistance as conditioned by Rps genes (rps, Rps1-k, Rps2, Rps3, Rps6) in two genetic backgrounds (Williams & Harosoy) on accumulation of soluble peroxidases were determined by a soybean peroxidase capture assay (SPCA) after inoculation with P. sojae races 2, 7, or 25.
Jose C, Melgar   +2 more
openaire   +2 more sources

Phytophthora sojae effectors orchestrate warfare with host immunity

Current Opinion in Microbiology, 2018
Phytophthora sojae is one of the most damaging plant pathogens of soybean. To aid establishment of a compatible interaction with its host, P. sojae deploys many secreted effectors. These effectors act either in the apoplastic space to cope with hostile conditions or inside of host cells to reprogram host physiology favoring pathogen growth.
Yan, Wang, Yuanchao, Wang
openaire   +2 more sources

Inheritance and mapping of 11 avirulence genes in Phytophthora sojae

Fungal Genetics and Biology, 2002
Two new crosses involving four races (races 7, 16, 17, and 25) of the soybean root and stem rot pathogen Phytophthora sojae were established (7/16 cross; 17/25 cross). An F2 population derived from each cross was used to determine the genetic basis of avirulence towards 11 different resistance genes in soybean.
May, K.J.   +7 more
openaire   +4 more sources

Glucan synthase of Phytophthora sojae

2002
Glucans, with the (1-3)-b-glucosidic linkage as major feature, are present in most of the higher plants, in many lower plants, as well as in microorganisms (Stone and Clarke, 1992). The synthesis of (1-3)-b-glucan in vivo is catalysed by the enzyme (1-3)-b-glucan synthase (EC 2.4.1.34; UDP-glucose:1,3-b-D-glucan 3-b-D-glucosyl transferase) using UDP ...
openaire   +1 more source

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