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Classical Genetics of Pichia pastoris
A significant advantage of P. pastoris as an experimental system and an eukaryotic expression platform is the ability to readily bring to bear both classical and molecular genetic approaches to a research problem and to develop technologies for manufacturing important proteins.Ilya, Tolstorukov +2 more
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Transformation of Pichia pastoris by Electroporation
Transformation is one of the cornerstone techniques of molecular biotechnology, and it is an almost obligatory part of any study related to the investigation of gene functions, the production of recombinant proteins, or the alterations of the phenotypic features in Komagataella phaffii.Kirill, Smirnov, Anton, Glieder
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Gene expression in yeast: Pichia pastoris
Current Opinion in Biotechnology, 1991Recent studies have shown the versatility and utility of the Pichia pastoris expression system. Improvements in strains have boosted the yield of proteins and peptides to the commercially feasible range. The Pichia pastoris expression system will soon be used to manufacture proteins for human clinical trials.
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Methods in enzymology, 2009
The peroxisome is an organelle whose quantity is tightly regulated in response to changes in metabolic status, and much knowledge has been accumulated regarding its dynamics. The turnover of peroxisomes through autophagic pathways, termed pexophagy, has been especially studied in several methylotrophic yeast strains capable of growth on methanol as a ...
Masahide, Oku, Yasuyoshi, Sakai
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The peroxisome is an organelle whose quantity is tightly regulated in response to changes in metabolic status, and much knowledge has been accumulated regarding its dynamics. The turnover of peroxisomes through autophagic pathways, termed pexophagy, has been especially studied in several methylotrophic yeast strains capable of growth on methanol as a ...
Masahide, Oku, Yasuyoshi, Sakai
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Sonolysis of Escherichia coli and Pichia pastoris in microfluidics
Lab Chip, 2012We report on an efficient ultrasound based technique for lysing Escherichia coli and Pichia pastoris with oscillating cavitation bubbles in an integrated microfluidic system. The system consists of a meandering microfluidic channel and four piezoelectric transducers mounted on a glass substrate, with the ultrasound exposure and gas pressure regulated ...
Tandiono, Tandiono +7 more
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ภาวะเหมาะที่สุดของการผลิตรีคอมบิแนนต์อินซูลินในยีสต์ Pichia pastoris KM71H
2023ปัจจุบันมีจำนวนของผู้ป่วยโรคเบาหวานเพิ่มสูงอย่างต่อเนื่องทั้งในระดับโลกและระดับชาติ ในประเทศไทย วิกฤตนี้ได้ส่งผลให้เกิดแรงผลักดันในการผลิตอินซูลินขึ้นภายในประเทศเอง ซึ่งมีจุดประสงค์เพื่อบำบัดรักษาผู้ป่วยโรคเบาหวาน และเพื่อหลีกเลี่ยงการนำเข้าอินซูลินจากต่างประเทศด้วย อินซูลินที่ใช้ในการบำบัดรักษาผู้ป่วยนั้นสามารถผลิตได้จากหลายวิธี อาทิ การสกัดจากตับอ ...
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Pichia pastoris Random Mutagenesis and Screening
Random mutagenesis provides a valuable tool to elucidate gene function and to draw connections between gene products and molecular pathways. This chapter provides two protocols for the random mutagenesis of Komagataella phaffii, along with methods to screen for the resulting mutant phenotypes.Christine, Winkler +2 more
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Expression of Foreign Genes in the yeast Pichia pastoris
2006Although the P. pastoris expression system is not suitable for the production of all foreign proteins, many are compatible with the system. For these proteins, there are major advantages to production in this yeast. Relative to higher eukaryotic expression systems, culturing costs are lower, yields (foreign protein/L of culture medium) are usually ...
G P, Lin Cereghino +3 more
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Heterologous Production of Fungal Effectors in Pichia pastoris
2011In this chapter a method for the heterologous production of fungal proteins in the yeast Pichia pastoris is described. Starting with cloning of the sequence encoding the gene of interest into the expression vector, this protocol describes P. pastoris transformation, production of the protein in a fermentor, and purification of the protein.
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