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Sub-THz spectroscopic characterization identification for pine wood nematode ribosomal DNA
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2020This paper, we introduced Sub-terahertz (Sub-THz) technology to identify nematode DNA sequence. First, data mining technology and restriction enzyme digestion were used to cut out two corresponding sequences, each containing about 100 base pairs that could represent the characteristic fragments of Bursaphelenchus xylophilus (Bx) and Bursaphelenchus ...
Fang Wang +5 more
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Nematology, 2005
AbstractSeveral methods were examined for extracting DNA from the woody tissues of Japanese black pine, including the general CTAB method which proved to be the most successful. To detect the pine wood nematode, the causal agent of pine wilt disease (PWD) in pine wood tissues, PWN-specific PCR was done using a DNA mixture obtained from 80 mg of pine ...
Kazuyoshi Futai +2 more
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AbstractSeveral methods were examined for extracting DNA from the woody tissues of Japanese black pine, including the general CTAB method which proved to be the most successful. To detect the pine wood nematode, the causal agent of pine wilt disease (PWD) in pine wood tissues, PWN-specific PCR was done using a DNA mixture obtained from 80 mg of pine ...
Kazuyoshi Futai +2 more
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European Journal of Wood and Wood Products, 2010
The research was aimed to establish whether dielectric heating at radio frequencies (RF)—an alternative method to ozone-depleting methyl bromide fumigation and to convective heating—could bring green lodgepole pine boards infested with pinewood nematodes (PWN) to pasteurization requirements.
Ciprian Lazarescu +4 more
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The research was aimed to establish whether dielectric heating at radio frequencies (RF)—an alternative method to ozone-depleting methyl bromide fumigation and to convective heating—could bring green lodgepole pine boards infested with pinewood nematodes (PWN) to pasteurization requirements.
Ciprian Lazarescu +4 more
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Parasitism Genes of the Pine Wood Nematode
2008Although the life history and some behavioral ecological aspects of Bursaphelenchus xylophilus, the pine wood nematode (PWN) are now well documented, little is known about the molecular basis of the nematodes’ biology and host-parasite interaction.
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Cortical responses in Japanese black pine to attack by the pine wood nematode
Canadian Journal of Botany, 1993Anatomical and cytochemical changes in the current-year stem cuttings of Japanese black pine (Pinus thunbergii) were investigated in the early stage of infection by a virulent isolate and an avirulent isolate of pine-wood nematode (Bursaphelenchus xylophilus), and an avirulent isolate of another nematode species, Bursaphelenchus mucronatus ...
Kyoko Ishida +3 more
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European Journal of Plant Pathology, 2001
Since the beginning of the 20th century, pine trees in Japan have been seriously damaged by the pine wilt disease. This disease is caused by the pine wood nematode, Bursaphelenchus xylophilus, which is transmitted by the Japanese pine sawyer, Monochamus alternatus.
Hajime Kosaka +4 more
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Since the beginning of the 20th century, pine trees in Japan have been seriously damaged by the pine wilt disease. This disease is caused by the pine wood nematode, Bursaphelenchus xylophilus, which is transmitted by the Japanese pine sawyer, Monochamus alternatus.
Hajime Kosaka +4 more
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Application of dsRNA in the Pine Wood Nematode, Bursaphelenchus xylophilus
The pine wood nematode Bursaphelenchus xylophilus is one of the most destructive invasive species worldwide, causing the wilting and eventual death of pine trees. Despite recognition of their economic and environmental significance, it has thus far been impossible to study the detailed gene functions of plant parasitic nematodes through conventional ...Chunyu, Wang, Kai, Guo
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Interactions of Pine Wood Nematodes, Wood-Inhabiting Fungi, and Vector Beetles
2008The pine wood nematode (PWN), Bursaphelenchus xylophilus, the causal agent of pine wilt disease (Kiyohara and Tokushige 1971), is vectored from wilt-killed to healthy pine trees by cerambycid beetles of the genus Monochamus (Mamiya and Enda 1972; Morimoto and Iwasaki 1972; Linit 1988) and kills healthy trees, but other organisms beside the PWN, pine ...
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Improved Pine Wood Nematode Disease Diagnosis System Based on Deep Learning
Plant DiseasePine wilt disease caused by the pine wood nematode, Bursaphelenchus xylophilus, has profound implications for global forestry ecology. Conventional PCR methods need long operating time and are complicated to perform. The need for rapid and effective detection methodologies to curtail its dissemination and reduce pine felling has become more apparent ...
Jiaming Xiao +6 more
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Host Fate Following Infection by the Pine Wood Nematode
2008Although the pine wood nematode (Bursaphelenchus xylophilus, PWN) seriously damages the physiology of its host pine trees, as described in Chaps. 21 and 22, it does not always kill host trees even when it successfully invades them. The main reasons for this are that both the virulence of the PWN and the susceptibility of pine trees to the nematode vary.
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