Results 41 to 50 of about 4,804,610 (291)
FEBS Letters, EarlyView.Transient oligomers formed by intrinsically disordered proteins may be ‘invisible’ to direct detection yet remain accessible to solution NMR through equilibrium‐exchange measurements and pressure‐jump experiments. Complementary methods report on mass, stoichiometry, selected distance distributions, morphology, and internal packing.
Martin D. Gelenter, Ad Bax
wiley +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
People and NatureStrengthening community engagement in stewardship can help to improve management of coastal and freshwater environments (blue spaces). Research suggests that recreational users of natural areas may be more willing to engage in stewardship.
Angela J. Dean +6 more
doaj +1 more source
The Impact of Socio-Demographic Factors on Tourism Experience: The Role of Sense of Place, Memorability, and Authenticity [PDF]
CactusTourists’ satisfaction and post-travel experiences are impacted by sense of place, memorability and authenticity. These concepts have been thoroughly studied, but not from a socio-demographic perspective.
Alina-Maria Vieriu +3 more
doaj +1 more source
FEBS Open Bio, EarlyView.NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks +4 more
wiley +1 more source
Determinants of brand love in wine experiences
Wine Economics and PolicyWine tourism provides a multisensory experience through various wine tourism activities. It also adds value to the regions and their producers by enhancing the wine knowledge and sensory experience of the visitors. This study explores the determinants of
Alvaro Dias +4 more
doaj +1 more source
FEBS Open Bio, EarlyView.Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
CONSUMERS SATISFACTION ANALYSIS OF OFFICE COFFEE IN BANJARBARU CITY, SOUTH KALIMANTAN PROVINCE, INDONESIA [PDF]
Russian Journal of Agricultural and Socio-Economic SciencesOffice Coffee is a coffee company established in 2015 under the umbrella of PT Indoharves. This study aims to assess consumer satisfaction with Office Coffee in Banjarbaru City.
Andriani E., Hamdani, Yanti N.D.
doaj
In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
FEBS Open Bio, EarlyView.Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source