Results 111 to 120 of about 253,025 (241)

Optimization of CRISPR-Cas9 system in Eustoma grandiflorum

iScience
Summary: The optimization of the CRISPR-Cas9 system for enhancing editing efficiency holds significant value in scientific research. In this study, we optimized single guide RNA and Cas9 promoters of the CRISPR-Cas9 vector and established an efficient ...
Xueqi Li, Fanqi Bu, Lishan Wang, Cholmin Kim, Wanjie Xue, Man Zhang, Saneyuki Kawabata, Qingzhu Zhang, Yuhua Li, Yang Zhang   +9 more
doaj   +1 more source

Improved the Activity of Phosphite Dehydrogenase and its Application in Plant Biotechnology. [PDF]

Front Bioeng Biotechnol, 2021
Liu T, Yuan L, Deng S, Zhang X, Cai H, Ding G, Xu F, Shi L, Wu G, Wang C.   +9 more
europepmc   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

From mouse to mouse-ear cress: Nanomaterials as vehicles in plant biotechnology. [PDF]

Exploration (Beijing), 2021
Xia X, Shi B, Wang L, Liu Y, Zou Y, Zhou Y, Chen Y, Zheng M, Zhu Y, Duan J, Guo S, Jang HW, Miao Y, Fan K, Bai F, Tao W, Zhao Y, Yan Q, Cheng G, Liu H, Jiao Y, Liu S, Huang Y, Ling D, Kang W, Xue X, Cui D, Huang Y, Cui Z, Sun X, Qian Z, Gu Z, Han G, Yang Z, Leong DT, Wu A, Liu G, Qu X, Shen Y, Wang Q, Lowry GV, Wang E, Liang XJ, Gardea-Torresdey J, Chen G, Parak WJ, Weiss PS, Zhang L, Stenzel MM, Fan C, Bush AI, Zhang G, Grof CPL, Wang X, Galbraith DW, Tang BZ, Offler CE, Patrick JW, Song CP.   +58 more
europepmc   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Designing a curriculum for Plant Biotechnology elective course for students in pre-university

Biotecnología Vegetal, 2013
To introduce the plant biotechnology in the curriculum of Vocational Pre-university Institutes of Sciences (IPVCE), increase the culture of the students in this specialty and motivate them to related careers, this study aimed to design a curriculum for ...
Alejandro G. Araújo Rodríguez
doaj  

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

FEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

Treatment with KCL‐286, a first‐in‐class retinoic acid receptor‐β (RARβ) agonist, ameliorates neuronal DNA damage and inflammation in a mouse model of Alzheimer's disease

FEBS Open Bio, EarlyView.
Repair of neuronal DNA damage in Alzheimer's disease by KCL‐286. (A) Amyloid‐β oligomers and plaques impair neuronal DNA repair pathways, leading to DNA double‐strand breaks and glial activation. (B) KCL‐286 activates RARβ/RXR signalling via retinoic acid response elements (RAREs), associated with increased BRCA1 expression, enhanced DNA repair and ...
Natasha Hill, Hassan Yousef Othman AlMuallim, Elouisa Maddock, Carl Hobbs, Earl Clarke, Maria B. Goncalves, Jonathan P. T. Corcoran   +6 more
wiley   +1 more source