Results 101 to 110 of about 252,861 (240)

Optimization of CRISPR-Cas9 system in Eustoma grandiflorum

iScience
Summary: The optimization of the CRISPR-Cas9 system for enhancing editing efficiency holds significant value in scientific research. In this study, we optimized single guide RNA and Cas9 promoters of the CRISPR-Cas9 vector and established an efficient ...
Xueqi Li, Fanqi Bu, Lishan Wang, Cholmin Kim, Wanjie Xue, Man Zhang, Saneyuki Kawabata, Qingzhu Zhang, Yuhua Li, Yang Zhang   +9 more
doaj   +1 more source

Improved the Activity of Phosphite Dehydrogenase and its Application in Plant Biotechnology. [PDF]

Front Bioeng Biotechnol, 2021
Liu T, Yuan L, Deng S, Zhang X, Cai H, Ding G, Xu F, Shi L, Wu G, Wang C.   +9 more
europepmc   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

From mouse to mouse-ear cress: Nanomaterials as vehicles in plant biotechnology. [PDF]

Exploration (Beijing), 2021
Xia X, Shi B, Wang L, Liu Y, Zou Y, Zhou Y, Chen Y, Zheng M, Zhu Y, Duan J, Guo S, Jang HW, Miao Y, Fan K, Bai F, Tao W, Zhao Y, Yan Q, Cheng G, Liu H, Jiao Y, Liu S, Huang Y, Ling D, Kang W, Xue X, Cui D, Huang Y, Cui Z, Sun X, Qian Z, Gu Z, Han G, Yang Z, Leong DT, Wu A, Liu G, Qu X, Shen Y, Wang Q, Lowry GV, Wang E, Liang XJ, Gardea-Torresdey J, Chen G, Parak WJ, Weiss PS, Zhang L, Stenzel MM, Fan C, Bush AI, Zhang G, Grof CPL, Wang X, Galbraith DW, Tang BZ, Offler CE, Patrick JW, Song CP.   +58 more
europepmc   +1 more source

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

Designing a curriculum for Plant Biotechnology elective course for students in pre-university

Biotecnología Vegetal, 2013
To introduce the plant biotechnology in the curriculum of Vocational Pre-university Institutes of Sciences (IPVCE), increase the culture of the students in this specialty and motivate them to related careers, this study aimed to design a curriculum for ...
Alejandro G. Araújo Rodríguez
doaj  

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

A transcriptome-wide identification of ATP-binding cassette (ABC) transporters revealed participation of ABCB subfamily in abiotic stress management of Glycyrrhiza glabra L

BMC Genomics
Transcriptome-wide survey divulged a total of 181 ABC transporters in G. glabra which were phylogenetically classified into six subfamilies. Protein–Protein interactions revealed nine putative GgABCBs (-B6, -B14, -B15, -B25, -B26, -B31, -B40, -B42 &-B44)
Ritu Devi, Pooja Goyal, Bhawna Verma, Shahnawaz Hussain, Fariha Chowdhary, Palak Arora, Suphla Gupta   +6 more
doaj   +1 more source

Robust Response to Plum pox virus Infection via Plant Biotechnology. [PDF]

Genes (Basel), 2021
Ravelonandro M, Briard P, Scorza R, Callahan A, Zagrai I, Kundu JK, Dardick C.   +6 more
europepmc   +1 more source

Acute caffeine treatment protects the developing retina from ischemia‐induced cell death

FEBS Open Bio, EarlyView.
Caffeine reduces cell death in the developing retina under ischemia (OGD). This effect does not involve BDNF upregulation or antioxidant pathways (NRF2/VEGF). Neuroprotection occurs mainly through adenosine A2A receptor antagonism, decreasing glutamate release and excitotoxicity, highlighting caffeine's potential as an acute neuroprotective agent in ...
Amanda Alves Nascimento, Danniel Pereira‐Figueiredo, Gabriella Grossi de Lima Dias, Gabriel Ferreira dos Santos, Mariana Rodrigues Pereira, Rafael Brito, Karin C Calaza   +6 more
wiley   +1 more source