Results 91 to 100 of about 473,806 (265)

Structure‐forward targeting of claudins with synthetic binders

FEBS Letters, EarlyView.
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley   +1 more source

Prediction of Bleeding via Simulation of Hydrodynamics in Centrifugal Partition Chromatography

Separations
Centrifugal Partition Chromatography (CPC) utilizes a two-phase liquid–liquid system as mobile and stationary phases. During operation, the latter continuously drains out of the rotor, despite it being in fact stationary, leading to decreasing separation
Felix Buthmann, Sophia Volpert, Jörg Koop, Gerhard Schembecker   +3 more
doaj   +1 more source

Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics

FEBS Letters, EarlyView.
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt, Ana‐Maria Sandru, Georg Vucak, Sebastian Didusch, Biljana Riemelmoser, Karin Ehrenreiter, Markus Hartl, Lukas A. Huber, Manuela Baccarini   +8 more
wiley   +1 more source

Comparative single-nucleus RNA-seq analysis revealed localized and cell type-specific pathways governing root-microbiome interactions

Nature Communications
Roots can recognize and differentially respond to beneficial and pathogenic microbes, which are fundamental for maintaining root microbiome homeostasis, plasticity, and plant fitness.
Qiuhua Yang, Zhuowen Li, Kaixiang Guan, Zhenghong Wang, Xianli Tang, Yechun Hong, Zhijian Liu, Jixian Zhai, Ancheng Huang, Yanping Long, Yi Song   +10 more
doaj   +1 more source

Optimizing Chromatographic Separation with Redosing: Effects on Separation Efficiency of a Model System in Centrifugal Partition Chromatography

Separations
This study investigates and optimizes chromatographic separation in a Centrifugal Partition Chromatograph. Therefore, a model system is separated in a single-disc rotor.
Felix Buthmann, Jan Hohlmann, Mareen Neuwald, Gerhard Schembecker   +3 more
doaj   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source

ROD1-controlled immune activation reshapes the rice phyllosphere microbiome

Phytopathology Research
The phyllosphere microbiome contributes to plant disease resistance, but how immune activation reshapes rice leaf-associated bacterial communities remains unclear.
Yuandong Wang, Haochen Zhang, Dongsheng Guo, Yue Wu, Haoyan Wang, Jirong Huang, Yiwen Deng, Zuhua He   +7 more
doaj   +1 more source

Designing with Plants [PDF]

HortTechnology, 2000
openaire   +1 more source

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source