Results 241 to 250 of about 475,966 (276)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source

An explainable deep learning framework for few shot crop disease detection in rice and sugarcane using CNN based feature extraction. [PDF]

Sci Rep
El-Behery H, Attia AF, Rezk NG.
europepmc   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

SRC-YOLOv8n: a lightweight framework for fine-grained apple leaf disease detection with spatial detail preservation and multi-scale feature enhancement. [PDF]

Front Plant Sci
Cui H, Song C, Zhong C, Du P, Xie L, Song Y, Li R, Jing X, Ouyang Q.   +8 more
europepmc   +1 more source

Editorial: Diagnostic innovations for microbial pathogens in edible plants: cutting-edge technologies for enhanced detection. [PDF]

Front Microbiol
Kumar R, Roy A, Zhai Y.
europepmc   +1 more source

Classification of rice plant diseases using efficient DenseNet121. [PDF]

Sci Rep
Ismail A, Hamdy W, Ibrahim AH, Awad WA.
europepmc   +1 more source

Lightweight deep learning for tomato disease detection: trends, challenges, and edge AI perspectives. [PDF]

Front Plant Sci
Gunasekaran H, Rajkumar S, Kirubhadharsini B L.   +2 more
europepmc   +1 more source

Chat Demeter: a multi-agent system for plant disease diagnosis integrating CNN-transformer models. [PDF]

Front Plant Sci
Zhang S.
europepmc   +1 more source