Results 101 to 110 of about 55,656 (302)

RNA-binding protein immunoprecipitation as a tool to investigate plant miRNA processing interference by regulatory proteins of diverse origin [PDF]

, 2018
Background: Due to the nature of viral RNA genomes, RNA viruses depend on many RNA-binding proteins (RBP) of viral and host origin for replication, dissemination and evasion of host RNA degradation pathways.
Garcia, Maria Laura, Marmisollé, Facundo Ernesto, Reyes Martinez, Carina Andrea   +2 more
core   +2 more sources

Emerging connections between small RNAs and phytohormones [PDF]

, 2020
Small RNAs (sRNAs), mainly including miRNAs and siRNAs, are ubiquitous in eukaryotes. sRNAs mostly negatively regulate gene expression via (post-)transcriptional gene silencing through DNA methylation, mRNA cleavage, or translation inhibition.
Dubois, Marieke, Gonzalez Sanchez, Nathalie, Inzé, Dirk, Li, Ting   +3 more
core   +2 more sources

KLK7 overexpression promotes an aggressive phenotype and facilitates peritoneal dissemination in colorectal cancer cells

FEBS Open Bio, EarlyView.
KLK7, a tissue kallikrein‐related peptidase, is elevated in advanced colorectal cancer and associated with shorter survival. High KLK7 levels in ascites correlate with peritoneal metastasis. In mice, KLK7 overexpression increases metastasis. In vitro, KLK7 enhances cancer cell proliferation, migration, adhesion, and spheroid formation, driving ...
Yosr Z. Haffani, Tobias Dreyer, Meriem Naim, Rea Lo Dico, Natalia A. Ignatenko, Viktor Magdolen, Dalila Darmoul   +6 more
wiley   +1 more source

Exploring the Diversity of Mechanisms Associated With Plant Tolerance to Virus Infection

Frontiers in Plant Science, 2018
Tolerance is defined as an interaction in which viruses accumulate to some degree without causing significant loss of vigor or fitness to their hosts. Tolerance can be described as a stable equilibrium between the virus and its host, an interaction in ...
Dinesh Babu Paudel, Hélène Sanfaçon
doaj   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Risk‐managed production of bioactive recombinant proteins using a novel plant virus vector with a helper plant to complement viral systemic movement

Plant Biotechnology Journal, 2010
SummaryA plant viral vector has the potential to efficiently produce recombinant proteins at a low cost in a short period. Although recombinant proteins can be also produced by transgenic plants, a plant viral vector, if available, may be more convenient when urgent scale‐up in production is needed.
Noriho, Fukuzawa, Takeaki, Ishihara, Noriko, Itchoda, Noriko, Tabayashi, Chiwa, Kataoka, Chikara, Masuta, Takeshi, Matsumura   +6 more
openaire   +2 more sources

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Viral Systemic Movement Is Enhanced by Alteration of a Structural Phloem Protein by the Insect Vector

Advanced Science
The ability to access and exploit the vascular system is essential for viral systemic spread within plant hosts. Insect‐borne viruses can be released from the vector's stylets into plant phloem together with saliva components that facilitate viral ...
Huijuan Guo, Qingyun Shi, Xiaoyu Ling, Yuting Wu, Lin He, Danyang Li, Keyan Zhu‐Salzman, Yucheng Sun   +7 more
doaj   +1 more source

Super‐Refractory Status Epilepticus (SRSE) in a Patient With Compound Heterozygous OPA1 Variants: Case Report and Literature Review

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Super‐Refractory Status Epilepticus (SRSE) is a rare, life‐threatening neurological emergency with unclear etiology in many cases. Mitochondrial dysfunction, often due to disease‐causing genetic variants, is increasingly recognized as a cause, with each gene producing distinct pathophysiological mechanisms.
Pouria Mohammadi, Lara Basovic, Christopher Michael McGraw   +2 more
wiley   +1 more source

Circulating virus load determines the size of bottlenecks in viral populations progressing within a host [PDF]

, 2012
For any organism, population size, and fluctuations thereof, are of primary importance in determining the forces driving its evolution. This is particularly true for viruses—rapidly evolving entities that form populations with transient and explosive ...
Blanc, Stéphane, Fereres, Alberto, Garzo, Elisa, Gutiérrez, Serafin, Michalakis, Yannis, Pirolles, Elodie, Yvon, Michel   +6 more
core   +5 more sources