In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
Correction: Differential response of finger millet accessions to contrasting saline water levels and irrigation regimes under desert conditions. [PDF]
Talabi A +14 more
europepmc +1 more source
Adenosine triphosphate as a modulator of protein interactions and stability
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley +1 more source
Physiological Indicators for Post-Translocation Monitoring of <i>Salix lapponum</i> in Natural vs. Degraded Peatlands. [PDF]
Arciszewski M, Pogorzelec M.
europepmc +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Organ-Dependent Antioxidant, Redox-Modulating, and Lifespan Effects of <i>Galanthus elwesii</i> Extracts in <i>Drosophila melanogaster</i>. [PDF]
Ertan O, Ayar A, Ay EB.
europepmc +1 more source
The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley +1 more source
Effects of Exogenous Gibberellic Acid (GA<sub>3</sub>) on Nitrogen Contents and Electrophysiological Parameters in Soybean (<i>Glycine max</i> (Linn.) Merr.) Under Drought Conditions. [PDF]
Xing D +6 more
europepmc +1 more source
A relationship of electrical impedance to water status of plant
Takahashi, Nobuhide +2 more
openaire +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source

