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Supercoil sequencing: a fast and simple method for sequencing plasmid DNA.
DNA, 1985A method for obtaining sequence information directly from plasmid DNA is presented. The procedure involves the rapid preparation of clean supercoiled plasmid DNA from small bacterial cultures, its complete denaturation by alkali, and sequence ...
E. Chen, P. Seeburg
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Plasmid Copy Number and Plasmid Stability
2003Many expression systems in research and industry use plasmids as vectors for the production of recombinant proteins or non-proteinous recombinant substances. Plasmids have an essential impact on productivity. Related factors are plasmid copy number, structural plasmid stability and segregational plasmid stability.
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Plasmid Segregation Mechanisms [PDF]
Annual Review of Genetics, 2005 Bacterial plasmids encode partitioning (par) loci that ensure ordered plasmid segregation prior to cell division. par loci come in two types: those that encode actin-like ATPases and those that encode deviant Walker-type ATPases. ParM, the actin-like ATPase of plasmid R1, forms dynamic filaments that segregate plasmids paired at mid-cell to daughter ...
Ebersbach, G., Gerdes, Kenn
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Molecular Microbiology, 1990
Recent development of vectors and methodologies to introduce recombinant DNA into members of the genus Mycobacterium has provided new approaches for investigating these important bacteria.
S. Snapper +4 more
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Recent development of vectors and methodologies to introduce recombinant DNA into members of the genus Mycobacterium has provided new approaches for investigating these important bacteria.
S. Snapper +4 more
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Plasmid-mediated resistance to vancomycin and teicoplanin in Enterococcus faecium.
New England Journal of Medicine, 1988THE glycopeptide antibiotic agents vancomycin and teicoplanin are useful in the treatment of severe infections due to gram-positive bacteria.1 2 3 Staphylococci, streptococci, and enterococci are a...
R. Leclercq +3 more
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2003
Bacterial plasmids are extra-chromosomal, covalently-closed circular (CCC) molecules of DNA that are capable of autonomous replication (1). Plasmids may contain genes for a variety of phenotypic traits, such as antibiotic resistance, virulence, or metabolic activities, although some plasmids comprise genes conferring no detectable phenotype and are ...
A P, Johnson, N, Woodford
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Bacterial plasmids are extra-chromosomal, covalently-closed circular (CCC) molecules of DNA that are capable of autonomous replication (1). Plasmids may contain genes for a variety of phenotypic traits, such as antibiotic resistance, virulence, or metabolic activities, although some plasmids comprise genes conferring no detectable phenotype and are ...
A P, Johnson, N, Woodford
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1985
It is a quarter century since the publications of evidence for the transmissibility of drug resistance among Enterobacteria (1,48). Although it was not the beginning of plasmid research, it was that discovery that led to the great expansion of work on plasmids, their natural history, and the uses to which they can be put. By their practical importance,
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It is a quarter century since the publications of evidence for the transmissibility of drug resistance among Enterobacteria (1,48). Although it was not the beginning of plasmid research, it was that discovery that led to the great expansion of work on plasmids, their natural history, and the uses to which they can be put. By their practical importance,
openaire +2 more sources