Results 101 to 110 of about 982,599 (302)

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Construction of a novel fungal gus expression plasmid, and its evaluation in Aspergillus nidulans : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Genetics at Massey University [PDF]

, 1996
A GUS expression plasmid, pFunGus, was constructed containing a multi-cloning site for the insertion of gene regulatory elements, to be used in fungal reporter gene studies.
McGowan, Tania Louise
core  

Open-source genomic analysis of Shiga-toxin–producing E. coli O104:H4 [PDF]

, 2011
An outbreak caused by Shiga-toxin–producing Escherichia coli O104:H4 occurred in Germany in May and June of 2011, with more than 3000 persons infected. Here, we report a cluster of cases associated with a single family and describe an open-source genomic
Antoine Danchin, Cordesmeyer S, Dongfang Li, Fei Pu, Feng Xi, George F. Gao, Holger Rohde, Huanming Yang, Itoh Y, Jian Wang, Jianguo Xu, Jun Oh, Jun Wang, Junhua Li, Junjie Qin, Kuijper E, Liang Yang, Mark J. Pallen, Martin Aepfelbacher, Martin Christner, Meiru Zhao, Moritz Hentschke, Nicholas J. Loman, Peng Wang, Robin Kobbe, Ruifu Yang, Scheutz F, Sebastian Loos, Shenghui Li, Wentong Chen, Xiangna Zhao, Xianwei Yang, Yajun Song, Yangqing Peng, Yin Li, Yingrui Li, Yuanlin Guan, Yujun Cui, Zhaoxi Zhang, Zhong Cen   +39 more
core   +1 more source

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

FEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source

Lipopolysaccharide is a frequent contaminant of plasmid DNA preparations and can be toxic to primary cells in the presence of adenovirus [PDF]

, 1994
Endotoxin (lipopolysaccharide, LPS) is commonly found as a contaminant in plasmid DNA preparations. We demonstrate here that the quantities of LPS typically contaminating DNA preparations can generate a toxicity to primary cells (primary human skin ...
Baker, A., Buschle, M., Cotten, Matt, Saltik, Mediyha, Wagner, Ernst   +4 more
core  

BMI‐1 modulation and trafficking during M phase in diffuse intrinsic pontine glioma

FEBS Open Bio, EarlyView.
The schematic illustrates BMI‐1 phosphorylation during M phase, which triggers its translocation from the nucleus to the cytoplasm. In cycling cells, BMI‐1 functions within the PRC1 complex to mediate H2A K119 monoubiquitination. Following PTC596‐induced M phase arrest, phosphorylated BMI‐1 dissociates from PRC1 and is exported to the cytoplasm via its
Banlanjo Umaru, Deepak Kumar Mishra, Shiva Senthil Kumar, Hao‐Han Pang, Brendan Devine, Komal Khan, Rachid Drissi   +6 more
wiley   +1 more source

Enhanced macrophage delivery to the colon using magnetic lipoplexes with a magnetic field

Drug Delivery, 2019
Magnetically guided cell delivery systems would be valuable to achieve effective macrophage-based cell therapy for colonic inflammatory diseases.
Yusuke Kono, Serika Gogatsubo, Takeshi Ohba, Takuya Fujita   +3 more
doaj   +1 more source

The cooperative regulation of miR‐221 by APE1 and AUF1 impacts p27Kip1 defining a miR signature relevant for cervical cancer

FEBS Open Bio, EarlyView.
A regulatory axis involving APE1, AUF1, and miR‐221 is proposed. Pri‐miR‐221 is processed by DROSHA and DICER to generate mature miR‐221, which targets p27Kip1 mRNA. APE1 and AUF1 compete for pre‐miR‐221 binding. Reduced APE1/AUF1 levels impair miR‐221 biogenesis, decrease p27Kip1 mRNA degradation, and promote cell cycle progression, chemoresistance ...
Matilde Clarissa Malfatti, Nicolò Gualandi, Gianluca Tell, Giulia Antoniali   +3 more
wiley   +1 more source

Substrate-source flexibility of an exponential-fed perfusion process to produce plasmid DNA for use as leishmaniasis vaccine

Biotechnology & Biotechnological Equipment, 2019
The use of plasmid DNA (pDNA) for human vaccines is a novel approach against leishmaniasis, a neglected tropical disease with severe clinical manifestations.
Aurora García-Rendón, Angelica García-Rendón, Roberto Guzmán, Armando Tejeda-Mansir   +3 more
doaj   +1 more source

Development of an efficient transformation system for Dothistromin pini : a thesis presented in partial fulfilment of the requirements for the degree of Masters of Science in Molecular Genetics at Massey University [PDF]

, 1996
A transformation system has been developed for the plant pathogenic fungus Dothistroma pini using a positive selection system based on the Escherichia coli hygromycin B phosphotransferase gene (hph).
Bidlake, Anita Therese
core