Results 151 to 160 of about 2,326,635 (307)

Conformational Switching of Proteins on Material Surfaces Enabled by Peptide‐Oriented Adsorption

Advanced Functional Materials, EarlyView.
Responsive biointerfaces could be achieved using conformationally responsive proteins; however, their interconversion can be lost upon surface adsorption. Herein, we demonstrate that the incorporation a Au binding peptide into calmodulin allows for binding control on Au wherein the protein retains conformational switching. This capability was confirmed
Sakthirupini Ramamurthy, Kyle B. Meerbott, Lorenzo Travaglini, Garima S. Dobhal, Artur Sawicki, Marc R. Knecht, Tiffany R. Walsh, Dominic J. Glover   +7 more
wiley   +1 more source

A Holistic Platform Approach for Developing Robust Pulmonary mRNA Delivery: Integrating Novel Phenolic Acid‐Derived Ionizable Lipids and Device Engineering

Advanced Functional Materials, EarlyView.
An integrated pulmonary mRNA delivery platform combining novel biodegradable syringic acid‐derived ionizable lipids, design‐of‐experiments formulation optimization, and vibrating‐mesh nebulizer engineering enabled stable aerosolization and efficient lung delivery.
Neha Kaushal, Younan Ma, Asad Khanmohammed, Dipen Parande, Hanming Zhang, Saket Dolare, Juliet Chen, Apiwat Wangweerawong, Saswata Karmakar, Hongfeng Deng, Frank Koppenhagen, Shraddha Sharma, Eric Reyes, Sherry He, Lianne Boeglin, Caroline Woo, Azadeh Paksa, Ayed Allawzi, Anusha Dias, Gregory Troiano, Shrirang Karve, Frank DeRosa   +21 more
wiley   +1 more source

An Accurate and Precise ddPCR-Based Method for Determining the Concentration of Plasmid DNA. [PDF]

Bio Protoc
Puleo F, Evans A, Mason C.
europepmc   +1 more source

An Epigenetic Fate Converter Based on Nuclei‐Targeting Lipid Nanoparticles Drives Neuronal Programming of Stem Cells for Spinal Cord Repair

Advanced Functional Materials, EarlyView.
Schematic illustration of LNP‐MPG nuclei‐targeting delivery of HMW‐FGF2 promoting histone acetylation to regulate the fate of DPSCs and treat spinal cord injury. LNPs components include pHMW‐FGF2 plasmid, DSPC, Dlin‐MC3‐DMA, cholesterol, and PEG2000, and are modified with MPG to form HMW‐FGF2@LNP‐MPG (HLM). HLM nuclei‐targets DPSCs to deliver HMW‐FGF2,
Heng Zhou, Qiang Qin, Xiqin Li, Hanyu Ding, Chun Xu, Yan He, Qingsong Ye   +6 more
wiley   +1 more source

Integrated Purification and Desalting of Plasmid DNA Using Monolith Columns. [PDF]

Electrophoresis
Miklavčič R, Sever T, Skrt P, Vodopivec Seravalli T, Štrancar A, Černigoj U.   +5 more
europepmc   +1 more source

Genetically Programmed Shape‐Morphing of Engineered Living Materials

Advanced Functional Materials, EarlyView.
Here, bacterial and mammalian engineered living materials (ELMs) are presented, in which living cells direct genetically programmed and enzymatically mediated shape‐morphing. The resulting deformation and shape‐recovery can be actuated in a genetically controlled manner by an ELM incorporating out‐of‐equilibrium counteracting biochemical reactions with
Jan Becker, Yuchen Liu, Miguel Baños, Rosanne Schmachtenberg, Mahmudul Hasan, Claudia Fink‐Straube, Luai R. Khoury, Wilfried Weber   +7 more
wiley   +1 more source

Engineering single-dose plasmid DNA for sustained in vivo delivery of designer incretins. [PDF]

Trends Biotechnol
Gary EN, Gao Y, Hojecki CE, Tursi NJ, Zhang W, Goldman AR, Laenger N, Tomirotti M, Khan MS, Sahai R, Oblander J, Huang J, Lin ZJ, Cui J, Liu X, Humeau L, Pallesen J, Parzych EM, Weiner DB.   +18 more
europepmc   +1 more source

Utilizing the Metastability of Spidroins as a Strategy for Improving Mechanical Properties of Biomimetic Spider Silk Fibers

Advanced Functional Materials, EarlyView.
Phase transitions are central for the assembly of recombinant silk proteins into fibers. Soluble protein can transition into condensed states such as condensates and gels. These states are metastable and evolve in a time‐dependent way. Metastability depends on the protein sequence, and this relationship can be used to enhance the mechanical properties ...
Fred‐Eric Sammalisto, Topias Lankinen, Celine Tallroth, Rafael A. Lara, Matilda Backholm, Markus B. Linder   +5 more
wiley   +1 more source

Quantitative Analysis of Plasmid DNA Distribution and Transgene Expression Following Cell Squeezing. [PDF]

Ann Biomed Eng
Sylvers J, Krakowiecki J, Wu Y, Yuan F.
europepmc   +1 more source

Remote Activation of Wnt Signaling and Cell Proliferation by E‐Cadherin Magnetomechanical Stimulation

Advanced Functional Materials, EarlyView.
A pioneering magnetogenetic toolkit enables non‐invasive, precise spatiotemporal modulation of intracellular Wnt/β‐catenin signaling. Functionalized magnetic nanoparticles (MNPs@E/EC15) target cellular E‐cadherins to generate localized magnetomechanical forces under low‐intensity magnetic fields.
Christian Castro‐Hinojosa, Pablo Martínez‐Vicente, Lucía G. Recaredo, Susel Del Sol‐Fernández, Pilar Gomollón‐Zueco, Yilian Fernández‐Afonso, Raluca M. Fratila, María Moros   +7 more
wiley   +1 more source