Results 161 to 170 of about 1,084,010 (405)

GDP‐fucose transporter SLC35C1: a potential regulatory role in cytosolic GDP‐fucose and fucosylated glycan synthesis

FEBS Open Bio, EarlyView.
The inactivation of SLC35C1 (GDP‐fucose transporter) and enzymes involved in GDP‐fucose biosynthesis was studied. Fucose supplementation increases the level of GDP‐fucose to abnormal, millimolar values in the absence of the TSTA3 protein and SLC35C1 in contrast to the GMDS/SLC35C1 double mutant.
Edyta Skurska, Mariusz Olczak
wiley   +1 more source

Replicon sequence typing of IncF plasmids carrying virulence and resistance determinants.

Journal of Antimicrobial Chemotherapy, 2010
OBJECTIVES IncF plasmids are frequently encountered in clinical enterobacterial strains associated with the dissemination of relevant antimicrobial resistance and virulence genes.
L. Villa, A. García-Fernández, D. Fortini, A. Carattoli   +3 more
semanticscholar   +1 more source

Effect of interleukin-22 on immunogenicity of DNA vaccine encoding TSA gene of leishmania major in BALB/c mice [PDF]

, 2014
Background and purpose: Previous Research shows the use of plasmids containing genes TSA to be useful as vaccines for Leishmania major. Recently, the role of interleukin-22 (IL-22) in tissue repair has been demonstrated.
Dalimi-Asl, A., Ghaffarifar, F., Niaz-Jorjani, O., Sharifi, Z., Ziaei-Hezarjaribi, H.   +4 more
core  

New Plasmid (pTU512), Mediating Resistance to Penicillin, Erythromycin, and Kanamycin, from Clinical Isolates of Staphylococcus aureus [PDF]

, 1978
Megumi Kono, Masanori Sasatsu, Kimiko Ubukata, Masatoshi Konno, Rhochi Fujii   +4 more
openalex   +1 more source

Construction of hyperthermostable d‐allulose 3‐epimerase from Arthrobacter globiformis M30 using the sequence information from Arthrobacter psychrolactophilus

FEBS Open Bio, EarlyView.
d‐Allulose can be produced from d‐fructose by d‐allulose 3‐epimerase. Based on sequence homology information, we successfully engineered thermostable mutants with the protein engineering method. By integrating positive mutations, we constructed an enzyme that exhibits hyperthermostability without a loss in the activity.
Kensaku Shimada, Kouhei Ohtani, Pushpa Kiran Gullapalli, Kazuhiko Ishikawa   +3 more
wiley   +1 more source

Biophysical characterization and ion transport with cell‐based and proteoliposome reconstitution assays of invertebrate K+‐Cl− cotransporters

FEBS Open Bio, EarlyView.
The K+‐Cl− cotransporters (KCCs) facilitate the symport of ions across the plasma membrane. They participate in physiological processes including neuronal regulation. Here, we characterized KCCs from Drosophila and Hydra vulgaris. Comparative analyses of transporters provide insights into the mechanism of KCC ion transport, regulation, and evolution ...
Satoshi Fudo, Marina Verkhovskaya, Coralie Di Scala, Claudio Rivera, Tommi Kajander   +4 more
wiley   +1 more source

Genomic Evolution of Two Acinetobacter baumannii Clinical Strains from ST-2 Clones Isolated in 2000 and 2010 (ST-2_clon_2000 and ST-2_clon_2010) [PDF]

, 2016
Acinetobacter baumannii is a successful nosocomial pathogen due to its ability to persist in hospital environments by acquiring mobile elements such as transposons, plasmids, and phages. In this study, we compared two genomes of A.
Blasco, L., Fernández García, L., Florido, J.P., Gato, E., López, M., Pachón Díaz, Jerónimo, Pascual Hernández, Álvaro, Rodriguez Baño, Jesus, Rueda, A   +8 more
core  

Bidirection replication from a unique origin in a mini-F plasmid. [PDF]

, 1977
Rudolf Eichenlaub, David H. Figurski, Donald R. Helinski   +2 more
openalex   +1 more source

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

FEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

Isolation and mapping of plasmids containing the Salmonella typhimurium origin of DNA replication. [PDF]

, 1979
Judith W. Zyskind, L T Deen, Douglas W. Smith   +2 more
openalex   +1 more source