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Plasmid Analysis

2003
Bacterial plasmids are extra-chromosomal, covalently-closed circular (CCC) molecules of DNA that are capable of autonomous replication (1). Plasmids may contain genes for a variety of phenotypic traits, such as antibiotic resistance, virulence, or metabolic activities, although some plasmids comprise genes conferring no detectable phenotype and are ...
A P, Johnson, N, Woodford
openaire   +2 more sources

Plasmid Vectors

2003
Genetic manipulation of mycobacteria has historically been difficult. This is in large part due to the impenetrable nature of the cell wall, resulting in difficulty both in introducing DNA into the bacterium and subsequent isolation of intact plasmid DNA.
N, Casali, S, Ehrt
openaire   +2 more sources

Plasmid Segregation Mechanisms

Annual Review of Genetics, 2005
Bacterial plasmids encode partitioning (par) loci that ensure ordered plasmid segregation prior to cell division. par loci come in two types: those that encode actin-like ATPases and those that encode deviant Walker-type ATPases. ParM, the actin-like ATPase of plasmid R1, forms dynamic filaments that segregate plasmids paired at mid-cell to daughter ...
Ebersbach G, Gerdes K
openaire   +4 more sources

Ti plasmids of Agrobacterium as conjugative plasmids

Nature, 1977
THE relationship of the plant-oncogenic properties of Agrobacterium tumefaciens to the presence in these strains of large plasmids (the Ti plasmids) has now been convincingly demonstrated in several ways: oncogenic strains cured of the Ti plasmid lose their oncogenicity irreversibly1–3; transfer of Ti plasmids to non-oncogenic plasmidless strains ...
C, Genetello   +5 more
openaire   +2 more sources

Plasmid Profile Typing and Plasmid Fingerprinting

2003
Plasmids are extra-chromosomal molecules of deoxyribonucleic acid (DNA) capable of autonomous replication. Such molecules have been identified in many bacterial genera and usually exist as covalently closed circular (CCC) molecules. Plasmids range in size from less than one megaDalton (MDa) to several hundred MDa.
E J, Threlfall, N, Woodford
openaire   +2 more sources

Degradative Plasmids

1983
This chapter describes the degradative plasmids. Microorganisms play a major role in the degradation of the products and by-products of the activities of animals and plants and other microorganisms. The failure of microorganisms to rapidly recycle any of these compounds results in their accumulation in the environment and can lead to the phenomenon of ...
openaire   +3 more sources

Plasmid Copy Number and Plasmid Stability

2003
Many expression systems in research and industry use plasmids as vectors for the production of recombinant proteins or non-proteinous recombinant substances. Plasmids have an essential impact on productivity. Related factors are plasmid copy number, structural plasmid stability and segregational plasmid stability.
openaire   +2 more sources

Plasmids

Endeavour, 1982
M, Day, N, Burton
openaire   +2 more sources

Beyond horizontal gene transfer: the role of plasmids in bacterial evolution

Nature Reviews Microbiology, 2021
Jerónimo Rodríguez-Beltrán   +2 more
exaly  

Two defence systems eliminate plasmids from seventh pandemic Vibrio cholerae

Nature, 2022
Milena Jaskólska   +2 more
exaly  

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