Results 61 to 70 of about 3,897,964 (375)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana +5 more
wiley +1 more source
Development of an aluminium filled epoxy insert using perfactory rapid prototyping technique and electroless nickel plating for low volume plastic injection moulding [PDF]
In this research, a rapid tool for low volume production plastic injection moulding is designed, developed, and tested on an injection moulding machine.
Rajaguru, Janaka Chandraguptha
core
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Background:. We aimed to summarize the distribution of pathogenic bacteria for postoperative infection of different genitourinary plastic surgery and the antimicrobial resistance of the major pathogens. Methods:.
Fengli Jiang, MM +6 more
doaj +1 more source
Development of a Material for Manufacturing a Mold for Plastic Injection [PDF]
قولبة الحقن هي عملية تستخدم لتصنيع كميات كبيرة من الحاويات البلاستيكية ذات الأشكال الهندسية المعقدة. على الرغم من استخدام CuBe دائمًا، إلا أن هناك حاليًا بعض السبائك البديلة مثل سبيكة CuNiSiCr التي تتميز بتكلفة تصنيع أقل وخصائص مماثلة. الهدف من هذه الورقة هو توصيف عملية الاندماج والمعالجات الميكانيكية الحرارية لسبيكة CuNiSiCr، من خلال تقنيات الفحص ...
Alfredo Carlos González +1 more
openaire +5 more sources
ABSTRACT Objective To identify inflammatory analytes in cerebrospinal fluid (CSF) and plasma associated with cognitive decline in cognitively normal (CN) older adults at risk for Alzheimer's disease (AD). Methods In a longitudinal study of 118 CN older adults (65–80 years, 54% APOE ε4, 26% preclinical AD), 1331 CSF and 1501 plasma analytes were ...
Jagan A. Pillai +13 more
wiley +1 more source
Facial Cosmetic Therapy Use Among Patients With Systemic Sclerosis: An Australian Cohort Study
Objective Systemic sclerosis (SSc) is associated with numerous facial manifestations for which patients may engage in cosmetic therapies. It is unclear how patients with SSc use these therapies. This study sought to characterize patient engagement and experiences with cosmetic therapies for SSc‐related and non‐SSc–related facial changes.
Zachary Warren +11 more
wiley +1 more source
Aesthetic patients in East Asia are commonly concerned about small apparent eye size. Simultaneous treatment of the glabellar and lateral canthal areas with botulinum neurotoxin has potential to provide improvements. This case series evaluated changes in
Maurício de Maio +6 more
doaj +1 more source

