Results 71 to 80 of about 138,754 (265)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Microstructural Characterization Of Quenched And Plastically Deformed Two-Phase α+β Titanium Alloys

Archives of Metallurgy and Materials, 2015
Development of microstructure in two-phase α+β titanium alloys is realized by thermomechanical processing – sequence of heat treatment and plastic working operations.
Motyka M., Sieniawski J., Ziaja W., Mrówka-Nowotnik G.   +3 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Development of Transmission Error Dynamic Testing Machine for Plastic Gear

Jixie chuandong, 2019
Based on the principle of single flank measurement, a dynamic testing machine for plastic gear transmission error is developed and can simulate the actual working condition of the plastic gear and realize the dynamic measurement of transmission error ...
Zhaoyao Shi, Xinli Ren, Bo Yu, Haizhou Li, Lintao Zhang, Yong Ye, Ping Li   +6 more
doaj  

Study on the evolution laws of overlying strata stress and mining-induced fracture zones under coordinated extraction in multi-working face areas

Frontiers in Earth Science
With the continuous increase in coal mining depth and intensity, the stress distribution in surrounding rock during multi-working face coordinated mining has become increasingly complex, significantly affecting the safe and efficient extraction of coal ...
Xinkuan Bai, Qiang Yuan, Qian Jiang, Qian Jiang, Jun Song, Zhu Chen, Xiaoliang Liu   +6 more
doaj   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source

Construction of Medical Quality Control Indicators System for Chinese Plastic and Aesthetic Major

Xiehe Yixue Zazhi
ObjectiveTo construct a quality control indicators system for Chinese plastic and aesthetic major and lay foundation for medical quality control.MethodsNational Quality Control Center of Plastic and Aesthetic Major established a working group in February
ZHANG Mingzi, SI Loubin, HUANG Jiuzuo, YU Nanze, ZHENG Jiaojie, CHEN Jie, WANG Xiaojun, LONG Xiao, XIONG Wei   +8 more
doaj   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Quantitative proteomic analysis reveals different characteristics of bladder cancer cells after exposure to bisphenol A

FEBS Open Bio, EarlyView.
Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu, Lanlan Li, Wenjuan Jia, Hanzhong Feng, Xiaoquan Yu, Youli Zhao, Fengzhi Yuan, Hui Ding, Junqiang Tian, Yong‐Xing He, Zhiping Wang   +10 more
wiley   +1 more source

Research on the evolution patterns of plastic zones in rock masses of multi-disturbance roadways and support control measures

Frontiers in Earth Science
The dynamic response characteristics of stress redistribution in roadway surrounding rock and the distribution patterns of plastic zones during different stages are pivotal to controlling rock mass stability.
Lingyun Zhang, Bo Lei, Yang Chen, Jin Ban, Xiying Zhang, Qiang Yao   +5 more
doaj   +1 more source