Results 181 to 190 of about 30,632 (260)

Tunneled Deep-plane Lift: A Novel Approach to Lower Facial and Neck Laxity. [PDF]

Plast Reconstr Surg Glob Open
Stanford N, Ramírez B, Varela A.
europepmc   +1 more source

Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy

Molecular Oncology, EarlyView.
We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley   +1 more source

Endoscopic gastric plication complications: early hemorrhagic event and late suture dehiscence. A two-case series. [PDF]

J Surg Case Rep
Torres Guaicha MV, Gordillo Calero AC, Tinoco Ortiz TL, Tovar-Cobos PM.   +3 more
europepmc   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

V-shaped capsular plication for treating cam-type femoroacetabular impingement with hip instability in borderline dysplastic hips: a retrospective cohort study. [PDF]

Front Surg
Lv Y, Zeng N, Guo D, Yang P, Liu H, Chen G, Li X, Lin D, Yang W.   +8 more
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Impact of Midline Rectovaginal Fascia Plication on Female Sexual Function and Surgical Outcomes: A Retrospective Study. [PDF]

J Clin Med
Uzun A, Bıyık İ, Yılmaz Z, Topçu G, Zeteroğlu Ü, Hatırnaz Ş, Sparić R, Tinelli A.   +7 more
europepmc   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Robotic single-port totally extraperitoneal repair and plication for ventral hernia with concomitant rectus diastasis: a case report. [PDF]

Int J Surg Case Rep
Chouihna H, Halabi M, Bishara E, Moussa H.   +3 more
europepmc   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source