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Plunge milling time optimization via mixed-integer nonlinear programming [PDF]
International audiencePlunge milling is a recent and efficient production mean for machining deep workpieces, notably in aeronautics. This paper focuses on the minimization of the machining time by optimizing the values of the cutting parameters ...
Bes, Christian +3 more
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Reproducible Sample Preparation of Virus-Infected Cells for Cryo-FIB/ET Using Manual Plunge Freezing [PDF]
Most viruses extensively remodel their host cells to establish productive infection. Visualization of virus-induced cellular remodeling by electron microscopy (EM) has been revolutionized in recent years by advances in cryo-focused ion beam (cryo-FIB ...
Nathalie Lavoie, Ekaterina Heldwein
doaj +2 more sources
Research of the Plunge Milling for Face Gear
Study the face gear machining method by using the plunge milling processing,according to the face gear slotting processing principle and gear engagement principle,a plunging processing method with multi group envelops processing is proposed.Based on the ...
Tang Jinyuan, Yang Xiaoyu
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Ultrastructure of human brain tissue vitrified from autopsy revealed by cryo-ET with cryo-plasma FIB milling [PDF]
Ultrastructure of human brain tissue has traditionally been examined using electron microscopy (EM) following fixation, staining, and sectioning, which limit resolution and introduce artifacts.
Benjamin C. Creekmore +4 more
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The Effect of Different Conditions on the Tensile Properties of AA7075-T6 During the Friction Stir Welding Process [PDF]
In this paper the investigation of the FSW result characteristics on AA7075-T6 of the highest grade is carried out using different process parameters. A vertical milling machine with different FSW tool geometry is used to weld AA7075.
A. Sharma +2 more
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Summary: Here, we present a protocol for assessing virus-infected cells using electron cryo-tomography (cryoET). It includes the basic workflows of seeding cells, plunge-freezing, clipping, cryo-focused ion beam milling (cryoFIB-milling), and cryoET, as ...
Linda E. Franken +3 more
doaj +1 more source
In the process of high-efficiency milling, due to the impact of plunge caused by the intermittent cutting of the milling cutter, there is a high frequency and small vibration between the milling cutter and the workpiece.
Peiyi Zhao +3 more
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In situ cryo-FIB/SEM Specimen Preparation Using the Waffle Method
Cryo-focused ion beam (FIB) milling of vitrified specimens is emerging as a powerful method for in situ specimen preparation. It allows for the preservation of native and near-native conditions in cells, and can reveal the molecular structure of protein ...
Oleg Klykov +7 more
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Tool geometry analysis for plunge milling of lead-free CuZn-alloys [PDF]
Plunge milling is a critical process step in mass manufacturing of rectangular shapes in electrical connector components. These shapes are manufactured by drilling a pilot hole and subsequent plunge milling with a radial offset (pitch) one or more times. The plunged cavity serves as guidance for the final broaching cut.
Baier, Stefan +3 more
openaire +2 more sources
Cryo-electron tomography (cryo-ET) is a formidable technique to observe the inner workings of vitrified cells at a nanometric resolution in near-native conditions and in three-dimensions. One consequent drawback of this technique is the sample thickness,
William Nicolas +2 more
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