Results 131 to 140 of about 1,283 (155)

A newly designed degenerate PCR primer based on pmoA gene for detection of nitrite-dependent anaerobic methane-oxidizing bacteria from different ecological niches

Applied Microbiology and Biotechnology, 2013
A new pmoA gene-based PCR primer set was designed for detection of nitrite-dependent anaerobic oxidation of methane (n-damo) bacteria from four different ecosystems, namely rice paddy soil, freshwater reservoir, reed bed, and sludge from wastewater treatment plant.
Ping Han, Gu Ji-Dong
exaly   +5 more sources

Spatiotemporal variations in the abundances of the prokaryotic rRNA genes, pmoA, and mcrA in the deep layers of a peat bog in Sarobetsu-genya wetland, Japan

Limnology, 2010
Spatiotemporal variations in microbial gene abundances were investigated to identify potential zones of methanotroph and methanogen biomass in a peat bog in Sarobetsu-genya wetland. The abundances of the bacterial and archaeal 16S rRNA genes, pmoA, and mcrA were 107–109, 107–108, 104–106, and 104–107 copies g−1 dry peat, respectively.
Takeshi Naganuma, Seiichiro Ioka, Naganuma Takeshi   +2 more
exaly   +2 more sources

Occurrence and diversity of nitrite-dependent anaerobic methane oxidation bacteria in the sediments of the South China Sea revealed by amplification of both 16S rRNA and pmoA genes

Applied Microbiology and Biotechnology, 2014
Nitrite-dependent anaerobic methane oxidation (n-damo) process is unique in linking the microbial carbon and nitrogen cycles, but the presence of n-damo bacteria in marine ecosystem and the associated environmental factors are still poorly understood. In the present study, detection of n-damo bacteria using 16S rRNA and pmoA gene-based PCR primers was ...
Jing Chen, Gu Ji-Dong
exaly   +5 more sources

Complex community of nitrite-dependent anaerobic methane oxidation bacteria in coastal sediments of the Mai Po wetland by PCR amplification of both 16S rRNA and pmoA genes

Applied Microbiology and Biotechnology, 2014
In the present work, both 16S rRNA and pmoA gene-based PCR primers were employed successfully to study the diversity and distribution of n-damo bacteria in the surface and lower layer sediments at the coastal Mai Po wetland. The occurrence of n-damo bacteria in both the surface and subsurface sediments with high diversity was confirmed in this study ...
Zhichao Zhou, Jing Chen, Gu Ji-Dong
exaly   +5 more sources

Phylogenetic characterization of endosymbionts of the hydrothermal vent mussel Bathymodiolus azoricus by analysis of the 16S rRNA, cbbL, and pmoA genes

Microbiology, 2006
In order to assess the phylogenetic diversity of the endosymbiotic microbial community of the gills of marine shellfish Bathymodiolus azoricus, total DNA was extracted from the gills. The PCR fragments corresponding to the genes encoding 16S rRNA, ribulose-bisphosphate carboxylase (cbbL), and particulate methane monooxygenase (pmoA) were amplified ...
Pimenov N V, Kuznetsov B B, B B Kuznetsov   +2 more
exaly   +3 more sources

Molecular phylogeny of type II methane-oxidizing bacteria isolated from various environments The GenBank accession numbers for the nearly complete 16S rRNA gene sequences for the isolates are AJ458466 to AJ458510. Partial sequences of the pmoA, mxaF and mmoX genes have been deposited under the accession numbers AJ458994–AJ459052, AJ459053–AJ459100 and AJ458511–AJ458535, respectively. Where multiple strains contained identical sequences, only one has been deposited.

Microbiology (United Kingdom), 2002
Pf Dunfield
exaly   +2 more sources

Diversity ofamoAandpmoAGenes in Extremely Saline Alkaline Soils of the Former Lake Texcoco

Geomicrobiology Journal, 2020
Ammonia-oxidizing bacteria (AOB) and aerobic methane oxidizing-bacteria (MOB) were studied in three extreme soils of the former Lake Texcoco, Mexico, with pH ranging from 8.5 to 10.5 and electrolyt...
Valentín Pérez-Hernández, Mario Hernández-Guzmán, Nancy Serrano-Silva, Marco Luna-Guido, Yendi Ebenezer Navarro-Noya, Joaquín Adolfo Montes-Molina, Luc Dendooven   +6 more
openaire   +1 more source

ARChip Epoxy and ARChip UV for covalent on-chip immobilization of pmoA gene-specific oligonucleotides

Analytical Biochemistry, 2004
ARChip Epoxy and ARChip UV are presented as novel chip platforms for oligonucleotide immobilization. ARChip Epoxy is made of reactive epoxy resin available commercially. ARChip UV consists of photoactivatable poly(styrene-co-4-vinylbenzylthiocyanate).
Claudia, Preininger, Levente, Bodrossy, Ursula, Sauer, Rudolf, Pichler, Alexandra, Weilharter   +4 more
openaire   +2 more sources

The evolution of the AMOA, PMOA and BMOA genes measured by ka/ks ratios.

Proceedings of the West Virginia Academy of Science, 2016
The copper-containing membrane-bound monooxygenase enzyme family consists of enzymes facilitating the oxidation of methane (catalyzed by particulate methane monooxygenase, pMMO) and ammonia (catalyzed by ammonia monooxygenase, AMO), in both aerobic and anaerobic methanotrophs and nitrifiers, respectively, and the oxidation of C2-10 alkanes (catalyzed ...
Evan Lau, Daniel Lukich, Tuan Le
openaire   +1 more source

Methanotrophic bacterial diversity in two diverse soils under varying land-use practices as determined by high-throughput sequencing of the pmoA gene

Applied Soil Ecology, 2017
Abstract Methanotrophic bacteria in soil serve as the only known biological sink of atmospheric methane, and impact net methane emission from soils. Most members of methanotrophs use particulate methane monooxygenase enzyme, encoded by the pmoA gene, to oxidize methane.
Aditi Sengupta, Warren A. Dick
openaire   +1 more source