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A newly designed degenerate PCR primer based on pmoA gene for detection of nitrite-dependent anaerobic methane-oxidizing bacteria from different ecological niches

Applied Microbiology and Biotechnology, 2013
A new pmoA gene-based PCR primer set was designed for detection of nitrite-dependent anaerobic oxidation of methane (n-damo) bacteria from four different ecosystems, namely rice paddy soil, freshwater reservoir, reed bed, and sludge from wastewater treatment plant.
P. Han, J. Gu
semanticscholar   +7 more sources

Complex community of nitrite-dependent anaerobic methane oxidation bacteria in coastal sediments of the Mai Po wetland by PCR amplification of both 16S rRNA and pmoA genes

Applied Microbiology and Biotechnology, 2014
In the present work, both 16S rRNA and pmoA gene-based PCR primers were employed successfully to study the diversity and distribution of n-damo bacteria in the surface and lower layer sediments at the coastal Mai Po wetland. The occurrence of n-damo bacteria in both the surface and subsurface sediments with high diversity was confirmed in this study ...
J. Chen, Zhichao Zhou, Ji-Dong Gu
semanticscholar   +7 more sources

Occurrence and diversity of nitrite-dependent anaerobic methane oxidation bacteria in the sediments of the South China Sea revealed by amplification of both 16S rRNA and pmoA genes

Applied Microbiology and Biotechnology, 2014
Nitrite-dependent anaerobic methane oxidation (n-damo) process is unique in linking the microbial carbon and nitrogen cycles, but the presence of n-damo bacteria in marine ecosystem and the associated environmental factors are still poorly understood. In the present study, detection of n-damo bacteria using 16S rRNA and pmoA gene-based PCR primers was ...
J. Chen, Zhichao Zhou, J. Gu
semanticscholar   +7 more sources

ARChip epoxy and ARChip UV for covalent on-chip immobilization of pmoA gene-specific oligonucleotides.

Analytical Biochemistry, 2004
ARChip Epoxy and ARChip UV are presented as novel chip platforms for oligonucleotide immobilization. ARChip Epoxy is made of reactive epoxy resin available commercially. ARChip UV consists of photoactivatable poly(styrene-co-4-vinylbenzylthiocyanate).
C. Preininger   +4 more
semanticscholar   +3 more sources

Diversity of amoA and pmoA Genes in Extremely Saline Alkaline Soils of the Former Lake Texcoco

Geomicrobiology Journal, 2020
Ammonia-oxidizing bacteria (AOB) and aerobic methane oxidizing-bacteria (MOB) were studied in three extreme soils of the former Lake Texcoco, Mexico, with pH ranging from 8.5 to 10.5 and electrolytic conductivity (EC) from 0.67 to 84.76 dS m−1, and in ...
Valentín Pérez-Hernández   +6 more
semanticscholar   +2 more sources

A rapid regulation with different response intensities of the pmoA gene guarantees process robustness towards methane surges in continuous and feast-famine bioreactors

Biochemical Engineering Journal, 2019
A rapid recovery of methanotrophic activity from process fluctuations such as surges in CH4 inlet load is of utmost importance in bioreactors to guarantee a robust abatement of this potent greenhouse gas.
E. Rodríguez   +6 more
semanticscholar   +2 more sources

Spatiotemporal variations in the abundances of the prokaryotic rRNA genes, pmoA, and mcrA in the deep layers of a peat bog in Sarobetsu-genya wetland, Japan

Limnology, 2011
Spatiotemporal variations in microbial gene abundances were investigated to identify potential zones of methanotroph and methanogen biomass in a peat bog in Sarobetsu-genya wetland. The abundances of the bacterial and archaeal 16S rRNA genes, pmoA, and mcrA were 107–109, 107–108, 104–106, and 104–107 copies g−1 dry peat, respectively.
M. Akiyama   +5 more
semanticscholar   +2 more sources

Newly designed primer pair revealed dominant and diverse comammox amoA gene in full-scale wastewater treatment plants.

Bioresource Technology, 2018
The discovery of complete ammonia oxidizing bacteria (CAOB) capable of performing the two-step nitrification process on their own has fundamentally upended our traditional perception.
Mingyuan Wang   +5 more
semanticscholar   +1 more source

Phylogenetic characterization of endosymbionts of the hydrothermal vent mussel Bathymodiolus azoricus by analysis of the 16S rRNA, cbbL, and pmoA genes

Microbiology, 2006
In order to assess the phylogenetic diversity of the endosymbiotic microbial community of the gills of marine shellfish Bathymodiolus azoricus, total DNA was extracted from the gills. The PCR fragments corresponding to the genes encoding 16S rRNA, ribulose-bisphosphate carboxylase (cbbL), and particulate methane monooxygenase (pmoA) were amplified ...
E M, Spiridonova   +3 more
openaire   +2 more sources

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