Results 121 to 130 of about 6,150,813 (282)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Quantitative determination of the leaching range of in-situ leaching mining area by stagnation point

open access: yesNuclear Engineering and Technology
The leaching range is critical to the leaching efficiency, production cost and environmental effect of in-situ leaching of uranium. In this study, the groundwater dynamics of the well-site was simulated to determine the leaching range in condition of ...
Chong Zhang   +7 more
doaj   +1 more source

Cardiac arrest in a 36 year-old after trigger point injection with lidocaine: Case report. [PDF]

open access: yesInterv Pain Med, 2022
Hattenbach J   +3 more
europepmc   +1 more source

De novo synthesis of short‐chain aldehydes and hydrocarbons secreted by the brown marmorated stink bug Halyomorpha halys

open access: yesFEBS Open Bio, EarlyView.
The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

CAE for Advanced Injection Molding Technologies

open access: yes, 2019
Injection molding is one of the most popular polymer processing methods and has been used to mass produce plastics products for many decades. However, due to increasingly stringent requirements and higher customer expectations, conventional injection ...
Chang, Rong-Yeu;Huang, Chao-Tsai (CT)
core   +1 more source

Design and Validation of a Multi-Point Injection Technology for MR-Guided Convection Enhanced Delivery in the Brain. [PDF]

open access: yesFront Med Technol, 2021
Prezelski K   +6 more
europepmc   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

open access: yesFEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti   +5 more
wiley   +1 more source

Trigger point injection for post-mastectomy pain: a simple intervention with high rate of long-term relief. [PDF]

open access: yesNPJ Breast Cancer, 2021
Khoury AL   +7 more
europepmc   +1 more source

BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells

open access: yesFEBS Open Bio, EarlyView.
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley   +1 more source

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