Results 61 to 70 of about 560,083 (295)

Mutant p53R273H disrupts PDPK1 homodimerization and contributes to PDPK1 activation

Molecular Oncology, EarlyView.
How mutant p53R273H drives AKT signaling is unclear. We show that p53R273H, but not wild‐type, directly binds PDPK1 via a mutation‐dependent conformational change. This interaction disrupts inhibitory PDPK1 homodimerization and enhances AKT phosphorylation.
Mei Chee Lim, Alvin Kunyao Guo, Yoko Itahana, Srinivasaraghavan Kannan, Valerie Goh, Mingmin Ong, Terina Yan Wen Tay, Weiying Ye, Sabhashina Radha Krishnan, Jun Nishiyama, Chandra S Verma, Koji Itahana   +11 more
wiley   +1 more source

Treatment of textile wastewater using chitosan and chitosan grafted acrylamide by microwave synthesis [PDF]

, 2013
Polymeric flocculants based on single chitosan and polyacrylamide grafted chitosan (Ch-g-PAM) have been successfully synthesized via microwave initiated method. Under optimal grafting conditions, 30.4% grafting has been observed in case of the microwave
Ngadi, Norzita, Roslan, Noor Azuin, Arsad, Nurul Jannah   +2 more
core  

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Water absorbent polymer in sugarcane crop Polímeros hidroabsorventes em cultura canavieira

Engenharia Agrícola, 2013
The water absorbent polymer effect on vegetative growth and production of Theoretical Recovery Sugar (TRS) of sugarcane cv. RB 86 7515 was evaluated on two field tests installed in randomized blocks, with four treatments and five repetitions. The polymer
Tadeu A. Marques, Patricia A. A. Marques, Marcos W. Suriani, Arleto T. dos Santos, Fernando C. Mendonça   +4 more
doaj  

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Polyacrylamide Regulated Phytohormone Balance and Starch Degradation to Promote Seed-Potato Sprouting and Emergence

Plants
Potatoes are typically seeded as tubers, and their slow sprouting significantly impacts production. Therefore, the effects of polyacrylamide (20 g·L−1, 30 g·L−1, and 40 g·L−1) as a seed potato dressing on sprouting, seedling growth, and biomass were ...
Meiqiang Yin, Dongmei Hu, Xiaohang Yu, Yijie Wang, Sa Song, Chongyue Wang, Qilin Hu, Yinyuan Wen   +7 more
doaj   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source

Heterogeneous electro-Fenton removal of polyacrylamide in aqueous solution over CoFe2O4 catalyst

Water Science and Technology
Polyacrylamide (PAM) in environmental water has become a major problem in water pollution management due to its high molecular mass, high viscosity and non-absorption by soil.
Shanshan Xu, Yi Yang, Fanxiu Li
doaj   +1 more source

Analysis of lipopolysaccharide antigens of Treponema hyodysenteriae [PDF]

, 1989
Lipopolysaccharide (LPS) extracts obtained from Treponema hyodysenteriae of serogroups A, B, D and E, and from T. innocens were examined by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), silver-staining, and immunoblotting with hyperimmune rabbit ...
Combs, B., Hampson, D.J., Mhoma, J.R.L.
core  

Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region

FEBS Open Bio, EarlyView.
Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson, Anja H. Schiemann, Evelyn Sattlegger   +2 more
wiley   +1 more source