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Preparative electrophoresis on polyacrylamide gel
Analytical Biochemistry, 1964Nickolas Calvanico, David Racusen
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Determination of protein phosphorylation by polyacrylamide gel electrophoresis
Journal of Microbiology, 2019Chang‐Ro Lee +4 more
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Neutral Polyacrylamide Gel Electrophoresis
Cold Spring Harbor Protocols, 2006Joseph Sambrook, David W. Russell
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One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE).
Methods in Enzymology, 2014J. Brunelle, R. Green
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RNA purification by preparative polyacrylamide gel electrophoresis.
Methods in Enzymology, 2013A. Petrov +3 more
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SDS Polyacrylamide Gel Electrophoresis of Proteins
1996Crosslinked polyacrylamide gels are formed from the polymerization of acrylamide monomer in the presence of smaller amounts of N,N'-methylene-bis-acrylamide (normally referred to as “bis-acrylamide”) (Fig. 1). Note that bis-acrylamide is essentially two acrylamide molecules linked by a methylene group and is used as a crosslinking agent.
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Electrophoresis of DNA in Nondenaturing Polyacrylamide Gels
2003For the majority of purposes, such as restriction mapping and sizing of cloned fragments, electrophoresis of DNA in horizontal agarose gels is perfectly adequate. As the size of the fragments of interest decreases to below 1000 base pairs (bp), however, the resolution deteriorates significantly, and an alternative is required.
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