Results 71 to 80 of about 6,525,667 (289)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Ring-Opening Copolymerization of ε-Caprolactone and L-Lactide Catalyzed by Aluminum Complexes [PDF]

E3S Web of Conferences
In recent years, poly(ε-caprolactone) (PCL) and poly (lactic acid) (PLA) have been widely adopted as biodegradable materials, with applications ranging from simple packaging to sophisticated biomedical devices.
Zhang Mengxue, Xiang Sen, Liu Shaofeng, Kan Ze   +3 more
doaj   +1 more source

Click chemistry in polymer science

Chem, 2023
R. Hoogenboom
semanticscholar   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

One-pot multi-step reactions based on thiolactone chemistry: A powerful synthetic tool in polymer science

, 2015
One-pot multi-step reactions based on thiolactone chemistry emerged as a powerful tool to prepare tailor-made, multi-functionalized polymer architectures in a one-pot and elegant manner. This feature article highlights the most important features of this
Pieter Espeel, F. D. Prez
semanticscholar   +1 more source

Quantitative proteomic analysis reveals different characteristics of bladder cancer cells after exposure to bisphenol A

FEBS Open Bio, EarlyView.
Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu, Lanlan Li, Wenjuan Jia, Hanzhong Feng, Xiaoquan Yu, Youli Zhao, Fengzhi Yuan, Hui Ding, Junqiang Tian, Yong‐Xing He, Zhiping Wang   +10 more
wiley   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Fluorescence Resonance Energy Transfer (FRET) Measurements in Polymer Science: A Review.

Macromolecular rapid communications, 2022
S. Valdez, M. Robertson, Zhe Qiang
semanticscholar   +1 more source

A Systematic Comparison of Alpha‐Synuclein Seed Amplification Assays for Increasing Reproducibility

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Seed amplification assays (SAAs) enable ultrasensitive detection of misfolded α‐synuclein across biofluids and tissues. Yet, heterogeneity in protocols limits cross‐study comparability and clinical translation. Here, we review α‐synuclein SAA methods and their performance across various biological matrices.
Manuela Amaral‐do‐Nascimento, Daniela F. Santos, Tuane C. R. G. Vieira, Tiago F. Outeiro   +3 more
wiley   +1 more source

Lithium-Ion Dynamic Interface Engineering of Nano-Charged Composite Polymer Electrolytes for Solid-State Lithium-Metal Batteries

Nano-Micro Letters
Highlights The surface charge characteristics of halloysite nanotubes (HNTs) are manipulated to engineer the Li+-dynamic interface (Li+-DI) in composite polymer electrolytes.
Shanshan Lv, Jingwen Wang, Yuanming Zhai, Yu Chen, Jiarui Yang, Zhiwei Zhu, Rui Peng, Xuewei Fu, Wei Yang, Yu Wang   +9 more
doaj   +1 more source