Novel Approaches to the Identification of Streptococcus pneumoniae as the Cause of Community-Acquired Pneumonia [PDF]
Current diagnostic tests lack sensitivity for the identification of the bacterial etiology of pneumonia. Attempts during the past 2 decades to improve sensitivity of detection of bacterial constituents in blood by use of antibody-antigen complexes and ...
Albrich, Werner C.+2 more
core
A simple method for direct cloning and sequencing cDNA by the use of a single specific oligonucleotide and oligo(dT) in a polymerase chain reaction (PCR) [PDF]
Martin W. Berchtold
openalex +1 more source
Microbubble‐Controlled Delivery of Biofilm‐Targeting Nanoparticles to Treat MRSA Infection
Here, an effective strategy using microbubble (MB)‐controlled delivery of biofilm‐targeting nanoparticles (BTNs) for removal and therapy of methicillin‐resistant Staphylococcus aureus (MRSA) biofilm infections is introduced. In vivo delivery of MB with BTN is demonstrated to silence key bacterial genes involved in biofilm formation (icaA), bacterial ...
Ju Yeon Chung+6 more
wiley +1 more source
Limits of Performance of Quantitative Polymerase Chain Reaction Systems [PDF]
Estimation of the DNA copy number in a given biological sample is an important problem in genomics. Quantitative polymerase chain reaction (qPCR) systems detect the target DNA molecules by amplifying their number through a series of thermal cycles ...
Hassibi, Arjang+2 more
core
Detection of HTLV‐I Genome in Seronegative Infants Born to HTLV‐I Seropositive Mothers by Polymerase Chain Reaction [PDF]
Shigeru Saito+9 more
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Thermoresponsive BrushGel Microcarriers for Efficient Cell Expansion and Enzyme‐Reduced Harvesting
Askari et al, report on temperature‐responsive microcarriers to minimize enzyme use and cost in dynamic cultures. Abstract Scaling up cell therapy requires efficient expansion of high‐quality cells. Microcarrier(MC)‐based systems offer high surface‐to‐volume ratios and reduce culture media usage.
Esfandyar Askari+9 more
wiley +1 more source
Probe directed at a segment of Rickettsia rickettsii rRNA amplified with polymerase chain reaction [PDF]
Kenneth H. Wilson+5 more
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Reihs et al. present the development of a humanized, animal‐free synovial membrane model for osteoarthritis. Methods include 3D biochip cultures of human‐primary OA synoviocytes with matrix and serum surrogates. Results show replication of synovial architecture and increased Yap1 expression.
Eva I. Reihs+17 more
wiley +1 more source