Results 301 to 310 of about 1,450,683 (312)

Identification of single specimens of the Anopheles gambiae complex by the polymerase chain reaction.

American Journal of Tropical Medicine and Hygiene, 1993
A ribosomal DNA-polymerase chain reaction (PCR) method has been developed for species identification of individuals of the five most widespread members of the Anopheles gambiae complex, a group of morphologically indistinguishable sibling mosquito ...
J. Scott, W. Brogdon, F. Collins
semanticscholar   +1 more source

Product differentiation by analysis of DNA melting curves during the polymerase chain reaction.

Analytical Biochemistry, 1997
A microvolume fluorometer integrated with a thermal cycler was used to acquire DNA melting curves during polymerase chain reaction by fluorescence monitoring of the double-stranded DNA specific dye SYBR Green I.
Kirk M. Ririe, R. Rasmussen, C. Wittwer
semanticscholar   +1 more source

A Universal Polymerase Chain Reaction Developer.

Angewandte Chemie, 2016
The versatility of PCR, the gold standard for amplification of DNA targets, is hampered by the laborious, multi-step detection based on gel electrophoresis. We propose a one-step, one-tube method for the rapid (5 min) naked-eye detection of PCR products,
P. Valentini, P. Pompa
semanticscholar   +1 more source

Group-specific primer and probe sets to detect methanogenic communities using quantitative real-time polymerase chain reaction.

Biotechnology and Bioengineering, 2005
Real-time polymerase chain reaction (PCR) is a highly sensitive method that can be used for the detection and quantification of microbial populations without cultivating them in anaerobic processes and environmental samples.
Youngseob Yu, Changsoo Lee, Jaai Kim, Seokhwan Hwang   +3 more
semanticscholar   +1 more source

Quantitative polymerase chain reaction.

British journal of hospital medicine, 2014
British Journal of Hospital Medicine, December 2014, Vol 75, No 12 © 2 01 4 M A H ea lth ca re L td Introduction Quantitative polymerase chain reaction (PCR), also known as real‐time PCR, is a technique used to quantify specific nucleic acid sequences in
James R. C. Miller, R. Andre
semanticscholar   +1 more source

Reverse transcription-polymerase chain reaction.

Cold Spring Harbor Protocols, 2014
Reverse transcription coupled to the polymerase chain reaction (RT-PCR) is commonly used to detect the presence of mRNAs, pre-mRNAs, or other types of RNA such as noncoding RNAs. The method involves using a primer annealed to the RNA of interest.
D. Rio
semanticscholar   +1 more source

Site-directed mutagenesis by overlap extension using the polymerase chain reaction.

Gene, 1989
Steffan N. Hoa, Henry D. Hunt, R. Horton, Jeffrey K. Pullen, Larry R. Peasea   +4 more
semanticscholar   +1 more source

Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.

Methods in Enzymology, 1987
K. Mullis, F. Faloona
semanticscholar   +1 more source

Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data

Neuroscience Letters, 2003
C. Ramakers, J. Ruijter, R. H. Deprez, A. Moorman   +3 more
semanticscholar   +1 more source

Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction.

Genomics, 1989
Masato Orita, Youichi Suzuki, T. Sekiya, K. Hayashi   +3 more
semanticscholar   +1 more source