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Current Protocols in Protein Science, 2002
AbstractThis appendix describes the methodology behind PCR and gives a method for amplifying DNA enzymatically by the PCR and for optimizing this reaction for the sequence and primer set of interest.
Martha F. Kramer, Donald M. Coen
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AbstractThis appendix describes the methodology behind PCR and gives a method for amplifying DNA enzymatically by the PCR and for optimizing this reaction for the sequence and primer set of interest.
Martha F. Kramer, Donald M. Coen
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QJM: An International Journal of Medicine, 1991
In a short time the PCR techniques has revolutionized research technology in many areas of medicine. Because of the ease and rapidity of the technique it is quickly becoming a standard clinical test for many diseases. Clinical applications continue to emerge from research labs and should rapidly expand to facilitate rapid medical diagnosis.
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In a short time the PCR techniques has revolutionized research technology in many areas of medicine. Because of the ease and rapidity of the technique it is quickly becoming a standard clinical test for many diseases. Clinical applications continue to emerge from research labs and should rapidly expand to facilitate rapid medical diagnosis.
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Journal of Chemical Education, 1993
It is difficult to underestimate the impact of the polymerase chain reaction (PCR) and related DNA amplification techniques on modern molecular biology and applied molecular medicine. PCR represents a rapid, sensitive, and specific method for amplification of nucleic acid sequences and is the basis for numerous molecular techniques that have become the
Juanita M. Villalobos, William C. Timmer
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It is difficult to underestimate the impact of the polymerase chain reaction (PCR) and related DNA amplification techniques on modern molecular biology and applied molecular medicine. PCR represents a rapid, sensitive, and specific method for amplification of nucleic acid sequences and is the basis for numerous molecular techniques that have become the
Juanita M. Villalobos, William C. Timmer
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Journal of Infectious Diseases, 1988
organism number to readily detectable levels, yet culture is not always easy or successful. A novel technique, the polymerase chain reaction (PCR), was recently developed for in vitro amplification of the DNA or RNA of an organism or gene defect, and culture may not be required.
Gerald Schochetman +2 more
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organism number to readily detectable levels, yet culture is not always easy or successful. A novel technique, the polymerase chain reaction (PCR), was recently developed for in vitro amplification of the DNA or RNA of an organism or gene defect, and culture may not be required.
Gerald Schochetman +2 more
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Infection Control and Hospital Epidemiology, 1991
Polymerase chain reaction is a simple method of DNA amplification which permits a single short sequence to be replicated in the Laboratory many million times. The procedure takes a few hours at most, and can be adapted for non-radioactive analysis. As little as a single human cel!, or single virus, can be studied.
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Polymerase chain reaction is a simple method of DNA amplification which permits a single short sequence to be replicated in the Laboratory many million times. The procedure takes a few hours at most, and can be adapted for non-radioactive analysis. As little as a single human cel!, or single virus, can be studied.
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2012
The polymerase chain reaction (PCR) has had a significant impact on all aspects of the molecular biosciences, from cancer research to forensic science. The sensitivity and specificity inherent in the technique allow minute quantities of genetic material to be detected while the unique properties of thermostable DNA polymerase ensure that abundant ...
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The polymerase chain reaction (PCR) has had a significant impact on all aspects of the molecular biosciences, from cancer research to forensic science. The sensitivity and specificity inherent in the technique allow minute quantities of genetic material to be detected while the unique properties of thermostable DNA polymerase ensure that abundant ...
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Journal of Clinical Immunology, 1989
The PCR, like recombinant DNA technology, has had an enormous impact in both basic and diagnostic aspects of molecular biology because it can produce large amounts of a specific DNA fragment from small amounts of a complex template. Recombinant DNA techniques create molecular clones by conferring on a specific sequence the ability to replicate by ...
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The PCR, like recombinant DNA technology, has had an enormous impact in both basic and diagnostic aspects of molecular biology because it can produce large amounts of a specific DNA fragment from small amounts of a complex template. Recombinant DNA techniques create molecular clones by conferring on a specific sequence the ability to replicate by ...
openaire +3 more sources