Results 301 to 310 of about 1,452,270 (349)
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Trends in Genetics, 1989
The polymerase chain reaction (PCR) is a powerful new method for 'in vitro cloning'. It can selectively amplify a single molecule of template DNA several millionfold in a few hours and has made possible new approaches to problems in molecular genetics, evolutionary biology, and development.
T J, White, N, Arnheim, H A, Erlich
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The polymerase chain reaction (PCR) is a powerful new method for 'in vitro cloning'. It can selectively amplify a single molecule of template DNA several millionfold in a few hours and has made possible new approaches to problems in molecular genetics, evolutionary biology, and development.
T J, White, N, Arnheim, H A, Erlich
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Journal of Infectious Diseases, 1988
organism number to readily detectable levels, yet culture is not always easy or successful. A novel technique, the polymerase chain reaction (PCR), was recently developed for in vitro amplification of the DNA or RNA of an organism or gene defect, and culture may not be required.
G, Schochetman, C Y, Ou, W K, Jones
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organism number to readily detectable levels, yet culture is not always easy or successful. A novel technique, the polymerase chain reaction (PCR), was recently developed for in vitro amplification of the DNA or RNA of an organism or gene defect, and culture may not be required.
G, Schochetman, C Y, Ou, W K, Jones
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POLYMERASE CHAIN REACTION STRATEGY
Annual Review of Biochemistry, 1992INTRODUCTION ..... . . . . . . . . . .. . . . . . . .... . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . .... . . . .. . ..... . .. . . THE BASIC METHOD . . .. . . . . . . . . . . ... .. . . . . . . . . . ..... . . . . . . . . . . ..... . . . . . . . . . . .... . . . . . . . . . . . . . . . . BIOCHEMISTRY OF THE REACTION .
N, Arnheim, H, Erlich
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Inverse Polymerase Chain Reaction
Nature Biotechnology, 1989We describe a procedure that extends the utility of the polymerase chain reaction (PCR) in allowing the geometric amplification of an unknown DNA sequence that flanks a core region of known sequence. DNA containing the core region is digested with appropriate restriction enzymes to produce a fragment of suitable size for PCR amplification.
H, Ochman +3 more
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Current Protocols in Protein Science, 2002
AbstractThis appendix describes the methodology behind PCR and gives a method for amplifying DNA enzymatically by the PCR and for optimizing this reaction for the sequence and primer set of interest.
Martha F, Kramer, Donald M, Coen
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AbstractThis appendix describes the methodology behind PCR and gives a method for amplifying DNA enzymatically by the PCR and for optimizing this reaction for the sequence and primer set of interest.
Martha F, Kramer, Donald M, Coen
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Journal of Clinical Immunology, 1989
The PCR, like recombinant DNA technology, has had an enormous impact in both basic and diagnostic aspects of molecular biology because it can produce large amounts of a specific DNA fragment from small amounts of a complex template. Recombinant DNA techniques create molecular clones by conferring on a specific sequence the ability to replicate by ...
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The PCR, like recombinant DNA technology, has had an enormous impact in both basic and diagnostic aspects of molecular biology because it can produce large amounts of a specific DNA fragment from small amounts of a complex template. Recombinant DNA techniques create molecular clones by conferring on a specific sequence the ability to replicate by ...
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Polymerase chain reaction engineering
Biotechnology and Bioengineering, 1997A mathematical model for polymerase chain reaction (PCR) is developed, taking into account the three steps in this process: melting of DNA; primer annealing; and DNA synthesis (polymerization). Activity and deactivation of the polymerase enzyme as a function of temperature is incorporated in the kinetic model to get a better understanding of the ...
J T, Hsu, S, Das, S, Mohapatra
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Polymerase chain reaction techniques
Current Opinion in Biotechnology, 1991Abstract Recent improvements to the polymerase chain reaction have produced greater priming specificity, better methods for the isolation of unknown DNA sequences, more efficient DNA recovery techniques, and new approaches to mutation detection and oligonucleotide synthesis.
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Clinics in Dermatology, 1991
In a short time the PCR techniques has revolutionized research technology in many areas of medicine. Because of the ease and rapidity of the technique it is quickly becoming a standard clinical test for many diseases. Clinical applications continue to emerge from research labs and should rapidly expand to facilitate rapid medical diagnosis.
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In a short time the PCR techniques has revolutionized research technology in many areas of medicine. Because of the ease and rapidity of the technique it is quickly becoming a standard clinical test for many diseases. Clinical applications continue to emerge from research labs and should rapidly expand to facilitate rapid medical diagnosis.
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2012
The polymerase chain reaction (PCR) has had a significant impact on all aspects of the molecular biosciences, from cancer research to forensic science. The sensitivity and specificity inherent in the technique allow minute quantities of genetic material to be detected while the unique properties of thermostable DNA polymerase ensure that abundant ...
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The polymerase chain reaction (PCR) has had a significant impact on all aspects of the molecular biosciences, from cancer research to forensic science. The sensitivity and specificity inherent in the technique allow minute quantities of genetic material to be detected while the unique properties of thermostable DNA polymerase ensure that abundant ...
openaire +2 more sources