Results 21 to 30 of about 19,674 (236)
Identification of more than two paternal haplotypes of the ovine fatty acid-binding protein 4 (FABP4) gene in half-sib families: evidence of intragenic meiotic recombination. [PDF]
PLoS ONE, 2014 The fatty acid binding protein 4 (FABP4) plays an important role in the regulation of lipid metabolism in mammals. In this study, two regions of ovine FABP4 spanning exon 2-intron 2 and exon 3-intron 3 were investigated in four hundred and twenty lambs ...
Wei Yan +4 more
doaj +1 more source
Nucleic acid stain-dependent single strand conformation polymorphisms [PDF]
BioTechniques, 2004 Single strand conformational poly-morphism (SSCP) is a mobility shift analysis of single-stranded DNA (ss-DNA) by nondenaturing polyacryl-amide gel electrophoresis to detect DNA sequence polymorphisms (1). Typical SSCP protocols detect single strands by autoradiography (2–4) or by treatment with a DNA stain after electrophoresis (5–12).
David R. McKee, M. Scott Thomson
openaire +3 more sources
High-Throughput Asymmetric-PCR SSCP Analysis Using Well-Controlled Temperature Conditions
BioTechniques, 1996 Single-stranded conformational polymorphism (SSCP) analysis is useful for mutation detection and polymorphism identification. To realize high-throughput SSCP analysis using a high-intensity electric field, the gel temperature must be well-controlled.
Masaharu Kiyama, Takeshi Fujita
doaj +1 more source
Revista Médica Clínica Las Condes, 2022 Resumen: Introducción: : El adenocarcinoma es actualmente el tipo más común de cáncer de pulmón. Las mutaciones en EGFR son predictivas de respuesta a TKI y son mutuamente excluyentes con mutaciones en KRAS.
José Miguel Clavero +7 more
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Emerging Infectious Diseases, 2001 The use of polymerase chain reaction-single-stranded conformational polymorphism (PCR-SSCP) to study rpoB gene mutations in rifampin-resistant (RIFr) Mycobacterium tuberculosis has yielded contradictory results.
Miriam Bobadilla-del-Valle +8 more
doaj +1 more source
Identification of Five Putative Yeast RNA Helicase Genes [PDF]
, 1990 The RNA helicase gene family encodes a group of eight homologous proteins that share regions of sequence similarity. This group of evolutionarily conserved proteins presumably all utilize ATP (or some other nucleoside triphosphate) as an energy source ...
Abelson, John +2 more
core +1 more source
Molecular dynamics as an approach to study prion protein misfolding and the effect of pathogenic mutations [PDF]
, 2011 Computer simulation of protein dynamics offers unique high-resolution information that complements experiment. Using experimentally derived structures of the natively folded prion protein (PrP), physically realistic dynamics and conformational changes ...
Daggett, Valerie, van der Kamp, Marc W
core +2 more sources
Structure of a rare non-standard sequence k-turn bound by L7Ae protein [PDF]
, 2014 Kt-23 from Thelohania solenopsae is a rare RNA kink turn (k-turn) where an adenine replaces the normal guanine at the 2n position. L7Ae is a member of a strongly conserved family of proteins that bind a range of k-turn structures in the ribosome, box C/D
Huang, Lin, Lilley, David M J
core +3 more sources
BioTechniques, 1996 Mitochondrial DNA (mtDNA) mutations have been implicated in an increasing number of human diseases. Many of these mutations are heteroplasmic and are only present at low levels in readily accessible human tissue such as blood.
Emma J. Sherratt +3 more
doaj +1 more source
Optimization of Nonisotopic PCR–Single-Strand Conformation Polymorphism Analysis [PDF]
Clinical Chemistry, 1997 PCR–single-strand conformation polymorphism (SSCP) analysis is an attractive technique used to screen for unknown mutations because of its simplicity and widespread applicability (1). The technique relies on single-nucleotide variations modifying the conformation of single-stranded DNA and therefore its mobility in polyacrylamide gels. The detection of
H, Blanché +2 more
openaire +2 more sources