Results 21 to 30 of about 5,544 (200)

The Generation of Successive Unmarked Mutations and Chromosomal Insertion of Heterologous Genes in Actinobacillus pleuropneumoniae Using Natural Transformation [PDF]

open access: yes, 2014
We have developed a simple method of generating scarless, unmarked mutations in Actinobacillus pleuropneumoniae by exploiting the ability of this bacterium to undergo natural transformation, and with no need to introduce plasmids encoding recombinases or
A Maas   +46 more
core   +11 more sources

Expert opinion on the identification, risk assessment, and mitigation of microorganisms and parasites relevant to xenotransplantation products from pigs

open access: yesXenotransplantation, Volume 30, Issue 5, September/October 2023., 2023
Abstract Xenotransplantation has the potential to address shortages of organs available for clinical transplantation, but concerns exist regarding potential risks posed by porcine microorganisms and parasites (MP) to the health of human recipients. In this study, a risk‐based framework was developed, and expert opinion was elicited to evaluate porcine ...
Huybert Groenendaal   +14 more
wiley   +1 more source

Development of a highly sensitive point‐of‐care test for African swine fever that combines EZ‐Fast DNA extraction with LAMP detection: Evaluation using naturally infected swine whole blood samples from Vietnam

open access: yesVeterinary Medicine and Science, Volume 9, Issue 3, Page 1226-1233, May 2023., 2023
The developed POCT is quicker and easier to perform and does not require special equipment. We validated a sensitive POCT for ASF in swine whole blood samples for field settings.This POCT is expected to facilitate early diagnosis and containment of ASF invasion into both regions in which it is endemic and eradicated.
Mai Thi Ngan   +11 more
wiley   +1 more source

Mycoplasma hyopneumoniae membrane protein Mhp271 interacts with host UPR protein GRP78 to facilitate infection

open access: yesMolecular Microbiology, Volume 118, Issue 3, Page 208-222, September 2022., 2022
Our previous study described a novel virulence mechanism of Mycoplasma hyopneumoniae that inhibits the host UPR to facilitate bacterial adherence and infection. The bacterial factors and mechanisms underlying this UPR modulation are unknown. Here we find that Mycoplasma hyopneumoniae membrane protein Mhp271 directly interacts with the host GRP78 (a ...
Qiao Pan   +4 more
wiley   +1 more source

Welfare of pigs on farm

open access: yesEFSA Journal, Volume 20, Issue 8, August 2022., 2022
Abstract This scientific opinion focuses on the welfare of pigs on farm, and is based on literature and expert opinion. All pig categories were assessed: gilts and dry sows, farrowing and lactating sows, suckling piglets, weaners, rearing pigs and boars. The most relevant husbandry systems used in Europe are described.
EFSA Panel on Animal Health and Welfare (AHAW)   +29 more
wiley   +1 more source

In vivo testing of novel vaccine prototypes against Actinobacillus pleuropneumoniae [PDF]

open access: yes, 2018
Actinobacillus pleuropneumoniae (A. pleuropneumoniae) is a Gram-negative bacterium that represents the main cause of porcine pleuropneumonia in pigs, causing significant economic losses to the livestock industry worldwide. A.
A Hensel   +55 more
core   +6 more sources

Investigation into an outbreak of Border disease virus in pigs in England

open access: yesTransboundary and Emerging Diseases, Volume 69, Issue 4, Page 1698-1706, July 2022., 2022
Abstract Border disease (BD) was first reported in 1959 in lambs from the border region of England and Wales. The causative virus (BD virus; BDV) has since been identified in several other ruminant species and pigs. The virus is prevalent in sheep flocks of UK, Europe and USA and has potential to inflict substantial economic losses.
Akbar Dastjerdi   +7 more
wiley   +1 more source

Vaccination of Cattle with the N Terminus of LppQ of Mycoplasma mycoides subsp. mycoides Results in Type III Immune Complex Disease upon Experimental Infection [PDF]

open access: yes, 2015
Contagious bovine pleuropneumonia (CBPP) is a serious respiratory disease of cattle caused by Mycoplasma mycoides subsp. mycoides. Current vaccines against CBPP induce short-lived immunity and can cause severe postvaccine reactions. Previous studies have
Brown RD   +7 more
core   +3 more sources

Proteomic and immunoproteomic insights into the exoproteome of Actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia

open access: yesMicrobial Pathogenesis, 2022
Porcine pleuropneumonia caused by Actinobacillus pleuropneumoniae affects pig health status and the swine industry worldwide. Despite the extensive number of studies focused on A. pleuropneumoniae infection and vaccine development, a thorough analysis of the A. pleuropneumoniae exoproteome is still missing.
Stelli G. Stancheva   +7 more
openaire   +2 more sources

Development of a novel high resolution melting assay for identification and differentiation of all known 19 serovars of Actinobacillus pleuropneumoniae

open access: yesMicrobiologyOpen, Volume 11, Issue 2, April 2022., 2022
Actinobacillus pleuropneumoniae infection is a worldwide problem in the pig industry. It is important to differentiate between the 19 serovars using an efficient molecular tool, thereby improving presently available diagnostic methods. Knowing the prevalent serovar, the right vaccines could be administered and pigs potentially carrying virulent strains
Simone Scherrer   +6 more
wiley   +1 more source

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