Gene-specific monitoring of T7-based RNA amplification by real-time quantitative PCR
BioTechniques, 2003 T7-based RNA amplification is applied when there is insufficient RNA. The overall extent of amplification can be measured spectrophotometrically (i.e., quantifying RNA yields), but this measurement does not provide information about the RNA amplification
Sandra G. Heil +4 more
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A pepper MSRB2 gene confers drought tolerance in rice through the protection of chloroplast-targeted genes. [PDF]
PLoS ONE, 2014 The perturbation of the steady state of reactive oxygen species (ROS) due to biotic and abiotic stresses in a plant could lead to protein denaturation through the modification of amino acid residues, including the oxidation of methionine residues ...
Joung Sug Kim +12 more
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A family with acute intermittent porphyria [PDF]
, 2008 Porphyrias are inherited defects in heme metabolism that result in excessive secretion of porphyrins and porphyrin precursors. Porphyrias can be classified into acute, (neuropsychiatric), cutaneous and mixed forms.
Billoo, Abdul Gaffar, Lone, Saira Waqar
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BioTechniques, 2002 Quantitative real-time or kinetic RTPCR is increasingly used for the quantification of specific mRNA targets, especially in clinical applications. To quantify the mRNA of cytokines and their receptors, which play important roles in the pathogenesis of ...
Benedikte Sabina Kühne +1 more
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Nitric oxide in fungi: is there NO light at the end of the tunnel? [PDF]
, 2016 Nitric oxide (NO) is a remarkable gaseous molecule with multiple and important roles in different organisms, including fungi. However, the study of the biology of NO in fungi has been hindered by the lack of a complete knowledge on the different ...
Cánovas López, David +3 more
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Cell Transplantation, 2014 Acute intermittent porphyria (AIP) is an autosomal dominant disorder characterized by insufficient porphobilinogen deaminase (PBGD) activity. When hepatic heme synthesis is induced, porphobilinogen (PBG) and 5-aminolevulinic acid (ALA) accumulate, which ...
Zhaohui Yin +5 more
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Development of highly sensitive and specific mRNA multiplex system (XCYR1) for forensic human body fluids and tissues identification. [PDF]
PLoS ONE, 2014 The identification of human body fluids or tissues through mRNA-based profiling is very useful for forensic investigations. Previous studies have shown mRNA biomarkers are effective to identify the origin of biological samples. In this study, we selected
Yan Xu +11 more
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Transient and intensive pharmacological immunosuppression fails to improve AAV-based liver gene transfer in non-human primates [PDF]
, 2012 BACKGROUND: Adeno-associated vectors (rAAV) have been used to attain long-term liver gene expression. In humans, the cellular immune response poses a serious obstacle for transgene persistence while neutralizing humoral immunity curtails re ...
Alfaro, C. (Carlos) +12 more
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The 5' untranslated region of the I factor, a long interspersed nuclear element-like retrotransposon of Drosophila melanogaster, contains an internal promoter and sequences that regulate expression [PDF]
, 1993 The I-R system of hybrid dysgenesis in Drosophila melanogaster is controlled by a long interspersed nuclear element-like retroposon, the I factor.
BUCHETON, A +3 more
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PORPHOBILINOGEN DEAMINASE AND ADENOSINE DEAMINASE ACTIVITY AS A POSSIBLE DIAGNOSTIC AID IN LYMPHATIC LEUKEMIAS [PDF]
Medical Journal Armed Forces India, 1999 It has been demonstrated recently that in addition to morphologic and immunologic methods, enzyme characterisation of lymphoid cells is useful in the study and classification of lympho proliferative disorders. Heme biosynthetic pathway and purine metabolism are reported to be disturbed in such patients, this study was an attempt to estimate two enzymes
Alka N, Sontakke, Y V, Sharma
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