Results 81 to 90 of about 99,109 (264)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

FEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay, Junjie Xu, Josep Rizo   +2 more
wiley   +1 more source

The $2$-$3$-Set Packing problem and a $\frac{4}{3}$-approximation for the Maximum Leaf Spanning Arborescence problem in rooted dags

, 2023
The weighted $3$-Set Packing problem is defined as follows: As input, we are given a collection $\mathcal{S}$ of sets, each of cardinality at most $3$ and equipped with a positive weight.
Neuwohner, Meike
core  

MagmaFlow: A desktop platform for artificial intelligence‐driven expression analysis

FEBS Open Bio, EarlyView.
MagmaFlow is a free, no‐code platform for gene expression analysis. It generates interactive volcano plots, links genes to literature, pathways, and diseases, prioritizes candidates using millions of publications, identifies affected biological processes, builds network diagrams, and exports publication‐ready figures and reports for macOS and Windows ...
Carlos E. Buss, Ao Li, Eduardo H. Gilglioni, Mayank Bansal, Sumeet Pal Singh, Latifa Bakiri, Alessandra K. Cardozo, Esteban N. Gurzov   +7 more
wiley   +1 more source

Contributions of rising mean river level of the Río de la Plata estuary on recorded changes in positive storm surges

Ocean and Coastal Research
A 117-year series of hourly-observed heights in the Río de la Plata estuary allows studying the recorded changes in positive storm surge (PSS) events spanning from 1905 to 2021, and calculate the annual mean river level (AMRL) trend. The analysis of PSS
M. Florencia de Azkue, Enrique E. D’Onofrio   +1 more
doaj   +1 more source

Brick partitions of graphs

, 2010
For each rational number q=b/c where b≥c are positive integers, we define a q-brick of G to be a maximal subgraph H of G such that cH has b edge-disjoint spanning trees, and a q-superbrick of G to be a maximal subgraph H of G such that cH−e has b edge ...
Jackson, Bill, Jordán, Tibor
core   +1 more source

Long‐Term Follow‐Up of Chemotherapy‐Associated Biological Aging in Women With Early Breast Cancer

Aging and Cancer, EarlyView.
Women threated with adjuvant chemotherapy for early breast cancer have sustained long‐term increase in p16INK4a,, a robust marker of cell senescence, suggesting a chemotherapy‐associated age acceleration. p16INK4a as well as other biomarkers may identify patients at greatest risk for senescence‐related diseases of aging.
Hyman B. Muss, Alexis C. Wardell, Allison M. Deal, Kirsten A. Nyrop, Allison Ross, Yara G. Abdou, Jeffrey D. Aldrich, Lisa A. Cary, E. Claire Dees, Katherine E. Reeder‐Hayes, Emily M. Ray, Natalia Mitin, Denis Tsygankov   +12 more
wiley   +1 more source

A Wavier Polar Jet Stream Contributed to the Mid‐20th Century Winter Warming Hole in the United States

AGU Advances
Winter waves in the polar jet stream are associated with extreme cold outbreaks and can modulate longer‐term winter temperature trends in the mid‐latitudes. Recent research has highlighted a positive trend in jet stream waviness from 1990 to 2010, with a
J. I. Chalif, E. C. Osterberg, T. F. Partridge   +2 more
doaj   +1 more source

CX3CL1 in Early Detection of Alzheimer's Disease: Plasma Dynamics Across Age and Disease Stages

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Backgrounds Alzheimer's disease (AD) is characterized by amyloid‐beta plaques, tau tangles, and neuroinflammation. C‐X3‐C motif chemokine ligand 1 (CX3CL1, also known as fractalkine), a neuroimmune chemokine implicated in AD pathogenesis, shows inconsistent alterations in plasma/serum across studies.
Ling Wang, Yujie Liu, Fei Li, Xuelin Li, Lanlan Li, Jie Zhang, Yali Xu   +6 more
wiley   +1 more source