Results 181 to 190 of about 14,945,351 (305)

Nano-SiO₂/Polymer Composite Gel Fluid-Loss Reducer for High-Temperature, Salt-Resistant Water-Based Drilling Fluids: Performance and Mechanism. [PDF]

Gels
Liu L, Liu J, Geng Y, Wang R, Meng B, Gao S, Li H, Xu Z, An Y, Sun Y.   +9 more
europepmc   +1 more source

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

FEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow, Isabel Brandtjen, Yaxin Mo, Melissa Gulley, Louis Osorio, Sagar Kudchodkar, Nandita C. Jhumur, Christine C. Roberts, Lisa K. Denzin, David I. Shreiber, Biju Parekkadan, Hao Lin, Joel N. Maslow   +12 more
wiley   +1 more source

Role of desolvation on biomolecular liquid-liquid phase separation. [PDF]

Elife
Zhang K, Peng Z, Li W, Wang W.
europepmc   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

High temperature leads to a loss of isohydry in Pinus radiata. [PDF]

J Exp Bot
Sharma K, Bourbia I, Pérez FR, Brodribb T.   +3 more
europepmc   +1 more source

P(NIPAM-<i>co</i>-DMAEMA) hydrogel engineering of magnetic nanodiscs for smart delivery of viral vectors under magnetic stimulation.

Chem Commun (Camb)
Macchione MA, Donnarumma PR, Hamdan F, Budić I, Gutiérrez-Pineda E, Kocar E, Marković T, Cerullo V, Gregurec D, Moya SE.   +9 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

From Suitability to Invasion Risk: Integrating Correlative and Mechanistic Models for Predicting the Potential Distribution of the Red Palm Weevil. [PDF]

Ecol Evol
Azrag AGA, Agboka KM, Abdel-Rahman EM, Gwokyalya R, Kemal SA, El-Shafie HAF, Bob MA, Mohamed SA, Ekesi S, Tenkouano A.   +9 more
europepmc   +1 more source