Results 71 to 80 of about 1,521,653 (264)
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
Design and analysis strategies for robust microbiome ageing research
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik +5 more
wiley +1 more source
Reconstructing enzyme evolution by protein engineering
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Investigating transcription factor dynamics in health and disease using FRAP
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source
Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta +3 more
wiley +1 more source
Interlaboratory Comparison of RF Power Measurements Made by Automatic Power Measurement Software
An interlaboratory comparison of RF power measurements using automatic power measurement software was carried out among partners of the European Partnership on Metrology project titled “Development of RF and microwave metrology capability II ...
Anil Cetinkaya +13 more
doaj +1 more source
k-Nearest Neighbors Algorithm in Profiling Power Analysis Attacks [PDF]
Power analysis presents the typical example of successful attacks against trusted cryptographic devices such as RFID (Radio-Frequency IDentifications) and contact smart cards.
Z. Martinasek +3 more
doaj
A regional key comparison EURAMET.EM-K5.1 and a supplementary comparison COOMET. EM-S2 for electrical power were conducted between participating laboratories from the Eurasian region.
Velychko Oleh, Karpenko Stanislav
doaj +1 more source
Structure‐forward targeting of claudins with synthetic binders
Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley +1 more source
Power-Efficiency Comparison of Spectrum-Efficient Optical Networks
With steady traffic volume growth in the core networks, it is predicted that the future optical network communication will be constrained mainly by the power consumption.
Sridhar Iyer
doaj +1 more source

