Results 81 to 90 of about 7,287,922 (257)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells

FEBS Open Bio, EarlyView.
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley   +1 more source

TRPML1 agonist ML‐SA5 attenuates pulmonary fibroblast activation by suppressing mTOR and restoring autophagic flux

FEBS Open Bio, EarlyView.
TGF‐β1 stimulation downregulates lysosomal channel TRPML1 in pulmonary fibroblasts. The TRPML1 agonist ML‐SA5 suppressed fibroblast‐to‐myofibroblast activation and collagen production. Mechanistically, ML‐SA5 inhibited mTOR phosphorylation, restored autophagic flux, and its effects were enhanced by rapamycin (mTOR inhibitor) and reversed by MHY1485 ...
Jiatong Yao, Xiaoting Kang, Lulu Meng, Shaoxuan Zhang, Qiujie Li, Jinyu Zhou, Mengyue Xue, Chu Li, Hua Zhu, Pengzhou Hang, Jing Zhao   +10 more
wiley   +1 more source

Chronobiology of Cancer: How Aging Fuels Oncogenesis at the Molecular Level

Aging and Cancer, EarlyView.
This graphical abstract illustrates the key biological pathways linking aging with cancer development and progression. In the upper left, cumulative exposure to ultraviolet radiation, toxins, and reactive oxygen species (ROS) causes DNA damage and genomic instability, whereas age‐related decline in repair mechanisms, such as ATM/ATR, BER, and NER ...
Anu Singh, Aroonima Misra, Sufian Zaheer
wiley   +1 more source

Empirical Risk Minimization for Probabilistic Grammars: Sample Complexity and Hardness of Learning [PDF]

, 2012
Probabilistic grammars are generative statistical models that are useful for compositional and sequential structures. They are used ubiquitously in computational linguistics.
Noah A. Smith, Smith, N. A., Shay B. Cohen, Cohen, S. B.   +3 more
core   +1 more source

A total loss minimization modulation for series-resonant dual-active-bridge dc–dc converter with triple phase shift control

Scientific Reports
In this paper, a Total Loss Minimization (TLM) modulation strategy is proposed to enhance the conversion performance of the series-resonant dual-active-bridge DC–DC converters.
Shengzhi Zhou, Jihong Wang, Jiahua Tang
doaj   +1 more source

Development of A Loss Minimization Based Operation Strategy for Embedded BTB VSC HVDC

Applied Sciences, 2019
Recently, there have been many cases in which direct current (DC) facilities have been placed in alternating current (AC) systems for various reasons. In particular, in Korea, studies are being conducted to install a back-to-back (BTB) voltage-sourced ...
Jaehyeong Lee, Minhan Yoon, Sungchul Hwang, Soseul Jeong, Seungmin Jung, Gilsoo Jang   +5 more
doaj   +1 more source

RNA Sequencing Resolves Cryptic Pathogenic Variants in Mitochondrial Disease

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Mitochondrial diseases are the most common inherited metabolic disorders, characterized by pronounced clinical and genetic heterogeneity that complicates molecular diagnosis. Although DNA‐based sequencing approaches have become standard in genetic testing, up to half of patients remain without a definitive diagnosis.
Zhimei Liu, Xin Duan, Fatemeh Peymani, Jia Wang, Chengjia Bao, Chaolong Xu, Ying Zou, Zixuan Zhang, Yunxi Zhang, Tongyue Li, Martin Pavlov, Junling Wang, Minhan Song, Tianyu Song, Xiaodi Han, Mingxi Sun, Danmin Shen, Ruoyu Duan, Huafang Jiang, Manting Xu, Holger Prokisch, Fang Fang   +21 more
wiley   +1 more source

TRANSMISSION LOSS MINIMIZATION USING ADVANCED UNIFIED POWER FLOW CONTROLLER (UPFC

, 2018
The capability of transmission loss minimization of the power system network by advanced unified power flow controller (UPFC) is the main focus in this paper. An important factor effecting power transmission systems today is power flow control.
Nida N Shaikh, Nazneen Choudhari,
core   +1 more source

Optimal Power Flow of a Power System Incorporating Stochastic Wind Power Based on Modified Moth Swarm Algorithm

IEEE Access, 2019
The combined heat and power (CHP) generator not only generates electrical power but also generates heat energy in a single process, which decreases the emission level significantly.
Ehab E. Elattar
doaj   +1 more source