Results 21 to 30 of about 4,914 (173)
In Vitro Growth of Preantral Follicle and Maturation of Intrafollicular Oocyte from Aged Mice
Journal of Animal Reproduction and Biotechnology, 2019 This study aimed to recover the ovarian function through in vitro culture of preantral follicles from aged mice. First, we isolated the preantral follicles from ovaries of sixty-seven-week old B6D2F1 mice with decreased fecundity to know how many ...
Jung-Ah Yoon, Jung-Kyu Choi
doaj +1 more source
In vitro development of pig preantral follicles [PDF]
Bioscientifica Proceedings, 2020 A limiting factor to realizing the full potential of many of the new reproductive techniques is the lack of abundant numbers of fertilizable oocytes. This problem could be addressed by using the large source of oocytes available from preantral and primordial follicles by developing systems for in vitro growth.
openaire +2 more sources
Journal of Animal Reproduction and Biotechnology, 2019 The objective of this study was to establish an in vitro culture system for ovarian preantral follicles of B6D2F1. First, we optimized the in vitro preantral-follicle culture by culture duration, follicle stimulating hormone (FSH) type, and activin A ...
Jung Kyu Choi
doaj +1 more source
Cryopreservation and characterization of canine preantral follicles
Cryobiology, 2018 The aim of this study was to define the population, morphological and ultrastructural characteristics of bitch preantral follicles (PAFs) and to compare the effects on the morphology of PAF of two cryopreservation techniques - slow freezing (SF) and vitrification (V) - of bitches' ovarian tissue. The average population (number per ovary) of PAFs was 48,
J.L.P.R. Jivago +5 more
openaire +2 more sources
Mechanical isolation of capuchin monkey (Cebus apella) preantral ovarian follicles
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 2003 The aim of this study was to adapt a mechanical procedure for the isolation of intact preantral follicles from Cebus apella ovaries. The interval effect of serial sections of the tissue chopper was tested on a number of preantral follicles isolated from ...
S.F.S. Domingues +6 more
doaj +1 more source
Cryopreservation of Preantral Follicles
, 2018 In mammals, the total number of female germ cells is already established by the time of birth, meaning that no mitosis will take place in oogonias thereafter. Their cryostorage, therefore, depends on ovarian tissue manipulation. As an alternative to mature oocyte cryopreservation, the maintenance of inactive preantral follicles is a remarkable option ...
Rivas Leonel, Ellen Cristina +2 more
openaire +4 more sources
Animals, 2023 This study evaluated the follicular atresia, cell proliferation, and anti-Mullerian hormone action in Aotus nancymae and Sapajus macrocephalus during three sexual phases (follicular, luteal, and gestational).
Deise de Lima Cardoso +5 more
doaj +1 more source
Methods for Equine Preantral Follicles Isolation: Quantitative Aspects
Reproduction in Domestic Animals, 2013 ContentsThe aim of this study was to test the use of mechanical and mechanical‐enzymatic methods, saline solution (SS), and PBS solution for the manipulation and isolation of mare ovarian preantral follicles (PAFs). The ovaries were subjected to mechanical isolation (mixer) alone or in association with enzymatic digestion (collagenase).
Rivas Leonel, Ellen +5 more
openaire +3 more sources
BMC Biotechnology, 2008 Background Cryopreservation of preantral follicles or ovarian tissues would enable the storage of large numbers of primordial follicles or preantral follicles and preserves the structural integrity of somatic and reproductive cells. In the present study,
Hsu Kung-Hao +5 more
doaj +1 more source
In vitro culture of bovine preantral follicles: a review [PDF]
Reproductive Biology and Endocrinology, 2014 Preantral follicles are the majority of the ovarian follicle population and their use as a source of homogeneous oocytes for bovine reproductive biotechnologies could result in a substantial advance in this field. However, while in other species embryos and offspring have been produced, in bovine species the results have been limited to the follicular ...
Araújo, Valdevane R +3 more
openaire +2 more sources