Results 41 to 50 of about 1,154 (169)

Raman Plus X: Biomedical Applications of Multimodal Raman Spectroscopy

Sensors, 2017
Raman spectroscopy is a label-free method of obtaining detailed chemical information about samples. Its compatibility with living tissue makes it an attractive choice for biomedical analysis, yet its translation from a research tool to a clinical tool ...
Nandan K. Das, Yichuan Dai, Peng Liu, Chuanzhen Hu, Lieshu Tong, Xiaoya Chen, Zachary J. Smith   +6 more
doaj   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Facile fabrication of functional PDMS surfaces with tunable wettablity and high adhesive force via femtosecond laser textured templating

AIP Advances, 2014
Femtosecond laser processing is emerged as a promising tool to functionalize surfaces of various materials, including metals, semiconductors, and polymers.
Yanlei Hu, Guoqiang Li, Jianwen Cai, Chenchu Zhang, Jiawen Li, Jiaru Chu, Wenhao Huang   +6 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Ionomycin suppresses cancer cell growth by disrupting mitochondrial transcription

FEBS Open Bio, EarlyView.
In this study, we identify a new function for the selective Ca2+ ionophore, ionomycin, as an inhibitor of mitochondrial transcription. Both total and nascent RNA analyses revealed that ionomycin treatment reduces the transcription of mitochondrial genes.
Lishen Wang, Mingqiang Deng, Dongmei He, Diqin Lai, Dongjun Liu, Dengyong Zhu, Zhenhua Zhang, Peng Tang, Chuanman Zhou, Menghui Yin, Xichen Bao   +10 more
wiley   +1 more source

Perfluorocarbon-Loaded Hydrogel Microcapsules from Interface Shearing for Magnetic Guided Ultrasound and Laser Activation

Frontiers in Physics, 2020
Stimuli-responsive microcarriers have received considerable attention in a variety of fields, including disease diagnosis, drug delivery, sensing, and imaging. Here, we report the generation of multiple-responsive perfluorocarbon-loaded magnetic hydrogel
Zhiqiang Zhu, Zhiqiang Zhu, Ming Zhang, Ming Zhang, Yuanqing Zhu, Yuanqing Zhu, Fangsheng Huang, Fangsheng Huang, Ting Si, Ting Si, Ronald X. Xu, Ronald X. Xu, Ronald X. Xu   +12 more
doaj   +1 more source

Exploring the influence of lactoferrin on wound healing and related signaling pathways: a systematic review

FEBS Open Bio, EarlyView.
Lactoferrin promotes wound healing by activating MAPK, PI3K/Akt, and extracellular matrix pathways, enhancing fibroblast remodeling, proliferation, and re‐epithelialization. These mechanisms highlight its therapeutic potential in inflammation control, tissue repair, and remodeling.
Morgana Lüdtke Azevedo, Kátia Cristiane Hall, Matheus dos Santos Fernandez, Geovanna Peter Corrêa, Rafael Guerra Lund   +4 more
wiley   +1 more source

An Adaptive Anti-Noise Neural Network for Bearing Fault Diagnosis Under Noise and Varying Load Conditions

IEEE Access, 2020
Fault diagnosis in rolling bearings is an indispensable part of maintaining the normal operation of modern machinery, especially under the varying operating conditions. In this paper, an end-to-end adaptive anti-noise neural network framework (AAnNet) is
Guoqiang Jin, Tianyi Zhu, Muhammad Waqar Akram, Yi Jin, Changan Zhu   +4 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Wayside Bearing Fault Diagnosis Based on a Data-Driven Doppler Effect Eliminator and Transient Model Analysis

Sensors, 2014
A fault diagnosis strategy based on the wayside acoustic monitoring technique is investigated for locomotive bearing fault diagnosis. Inspired by the transient modeling analysis method based on correlation filtering analysis, a so-called Parametric ...
Fang Liu, Changqing Shen, Qingbo He, Ao Zhang, Yongbin Liu, Fanrang Kong   +5 more
doaj   +1 more source