Results 231 to 240 of about 26,519,848 (254)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

A multicentre study for clinical phenotype prediction in juvenile dermatomyositis: categorical principal component analysis-based hierarchical clustering. [PDF]

Reumatologia
Torun R, Cüceoğlu MK, Arslanoğlu Aydın E, Arık SD, Günalp A, Ulu K, Köse H, Yıldız Ç, Şahin N, Baba Ö, Açarı C, Öztürk K, Kılbaş G, Adıgüzel Dündar H, Yüksel S, Kalyoncu M, Sönmez HE, Bakkaloğlu S, Kılıç SŞ, Sözeri B, Kasapçopur Ö, Ayaz NA, Bağlan E, Bilginer Y, Keskinoğlu P, Ünsal E.   +25 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Accurate iodine quantification and residual error reduction with principal component analysis multimaterial decomposition using spectral CT. [PDF]

Med Phys
Khodajou-Chokami H, Ding H, Molloi S.
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Influencing Factors of Pine Wood Milling Force Based on Principal Component Analysis and Multiple Linear Regression. [PDF]

Materials (Basel)
Shen B, Buck D, Yuan Z, Zhu Z.
europepmc   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Identifying risk patterns for sudden cardiac death in athletes: A clustering and principal component analysis approach. [PDF]

PLoS One
Sgarro GA, Vasco P, Santoro D, Grilli L, Giglio M, Brunetti ND, Traetta L, Cibelli G, Valenzano AA.   +8 more
europepmc   +1 more source

Combination of partial least square structural equation modeling scheme of principal component analysis with importance performance analysis. [PDF]

MethodsX
Otok BW, Alfasanah Z, Aksioma DF.
europepmc   +1 more source

Within-sibling attenuation of polygenic risk score accuracy: investigating the effects of principal component analysis, LD score regression, and mixed model association in the UK Biobank. [PDF]

Hum Genet
Kelly CM, Onuorah O, Gilbert E.
europepmc   +1 more source