Results 271 to 280 of about 1,534,564 (323)

An enzyme‐linked immunosorbent assay (ELISA)‐based activity assay for AMP‐activated protein kinase (AMPK)

FEBS Open Bio, EarlyView.
Measuring AMP‐activated protein kinase (AMPK) activity in vitro is crucial for testing AMPK activators or inhibitors with therapeutic potential. Here, we report an enzyme‐linked immunosorbent assay (ELISA)‐based AMPK activity assay with simple steps and high sensitivity, which offers a simple, robust, and cost‐effective alternative to traditional ...
Trezze P. Nguyen, Shangze Lyu, Yang Liu
wiley   +1 more source

Bioengineering facets of the tumor microenvironment in 3D tumor models: insights into cellular, biophysical and biochemical interactions

FEBS Open Bio, EarlyView.
The tumor microenvironment is a dynamic, multifaceted complex system of interdependent cellular, biochemical, and biophysical components. Three‐dimensional in vitro models of the tumor microenvironment enable a better understanding of these interactions and their impact on cancer progression and therapeutic resistance.
Salma T. Rafik, Deniz Bakkalci, Alexander J. MacRobert, Umber Cheema   +3 more
wiley   +1 more source

Effect of Rapid Thermal Annealing on the Characteristics of Micro Zn-Doped Ga₂O₃ Films by Using Mixed Atomic Layer Deposition. [PDF]

Nanomaterials (Basel)
Tao J, Jiang X, Fan A, Hu X, Wang P, Dong Z, Wu Y.   +6 more
europepmc   +1 more source

Hydrophobicity causes anomalous migration of cystine/glutamate antiporter SLC7A11 in SDS‐PAGE with low acrylamide concentration

FEBS Open Bio, EarlyView.
SLC7A11 frequently migrates faster in SDS‐PAGE. The present study found that the high hydrophobicity of SLC7A11 causes its anomalous migration in SDS‐PAGE with a low concentration of acrylamide gel. Replacing isoleucine with asparagine reduced hydrophobicity and restored its normal migration at 55 kDa, revealing the role of hydrophobicity and gel ...
Nsengiyumva Emmanuel, Qian He, Yixin Kang, Dianbao Zhang, Min Gao, Minglin Wang, Kexin Fan, Jingwen Xiong, Shaobo Wu, Botao Fa, Zhengtao Xiao, Yingfang Niu, Jun Yao, Yilei Zhang   +13 more
wiley   +1 more source

Short-loop engineering strategy for enhancing enzyme thermal stability. [PDF]

iScience
Zhu W, Liu Y, Cao H, Liu L, Tan T.
europepmc   +1 more source

Comparative single‐cell transcriptomic profiling of patient‐derived renal carcinoma cells in cellular and animal models of kidney cancer

FEBS Open Bio, EarlyView.
We generated and characterized clear cell renal cell carcinoma models using the patient‐derived RCC243 cell line—including cell culture, orthotopic, and metastatic tumors—via single‐cell RNA‐sequencing for comparisons between models and patient tumor datasets.
Richard Huang, Lynn Kee, Alexander Gont, Jalna Meens, Fraser G. Ferens, Meredith S. Irwin, Laurie Ailles, Scott A. Yuzwa, Claire M. Robinson, Michael Ohh   +9 more
wiley   +1 more source

Second harmonic generation and nonlinear frequency conversion in photonic time-crystals. [PDF]

Light Sci Appl
Konforty N, Cohen MI, Segal O, Plotnik Y, Shalaev VM, Segev M.   +5 more
europepmc   +1 more source

Redesigning FDM Platforms for Bio-Printing Applications. [PDF]

Micromachines (Basel)
Turker B.
europepmc   +1 more source

Evolution of Biological Hydroxyapatite Modification Strategy: Anti-Inflammation Approach Rescues the Calcium-NOD-Like Receptor-Inflammation Axis-Mediated Periodontal Redevelopment Failure. [PDF]

Biomater Res
Zhou X, Xue J, Zhang Y, Xia R, Shan Z, Zhang L, Gui M, Liu G, Chen Z.   +8 more
europepmc   +1 more source

Study on the two-phase coupling migration mechanism of deceleration aggregate and water in coal mine water inrush channel. [PDF]

Sci Rep
Wen J, Gu S, Su P, Li J.
europepmc   +1 more source