Results 181 to 190 of about 847,146 (267)

Decoupling Sugar Availability and Metabolic Efficiency Reveals Bottlenecks in Ethanol Production from Lignocellulosic Hydrothermal Hydrolysates. [PDF]

ACS Omega
Vezaro FD, Schulz A, da Rosa BV, Garcia CMS, Kuhn RC, Teixeira JAC, Abaide ER, Mayer FD.   +7 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Isolation to purification: An integrated study on lysine production by Bacillus megaterium NTJT4 from medicinal plant rhizosphere. [PDF]

PLoS One
Jannat T, Karim MR, Hasan MR, Karmakar D, Tasnim N, Tarannum N.   +5 more
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Grey relational analysis-based optimisation of bioethanol production process parameters derived from cassava peels. [PDF]

Biotechnol Notes
John JY, Kulla DM, Narayan S, Said MFM, Abubakar S.   +4 more
europepmc   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Early Flowering and Rapid Growth Become Advantageous over Non-Lodging Plants in Field Pea Under Early Drought. [PDF]

Life (Basel)
Arinov B, Khassanova G, Kuzbakova M, Jatayev S, Kanatkyzy M, Kylyshbayeva G, Sweetman C, Shavrukov Y.   +7 more
europepmc   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Enteric Methane Emission and Milk Component Profiles of Holstein and Jersey Cows: A Respiration Chamber Study. [PDF]

Animals (Basel)
Park J, Eom JS, Kim DH, Park SM, Han SB, Kim Y, Dang CG, Lim DH.   +7 more
europepmc   +1 more source

UiO‐66 metal–organic frameworks in biomedicine: From structural tunability to bioimaging, photodiagnostics, and photodynamic cancer therapy

FEBS Open Bio, EarlyView.
UiO‐66(Zr) metal–organic frameworks are chemically stable, biocompatible, and highly tunable nanomaterials. Their modular structure enables controlled drug delivery, multimodal bioimaging, and light‐activated photodynamic therapy, supporting integrated diagnostic and therapeutic (theranostic) applications in cancer and biomedical research.
Veronika Huntošová, Grigorii Rakhalskii, Miroslav Almáši   +2 more
wiley   +1 more source