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Radioimmunoassay of the F Prostaglandins [PDF]

The Journal of Clinical Endocrinology & Metabolism, 1971
ABSTRACT Antibodies to prostaglandin F2α (PGF2α) were produced in rabbits immunized with a conjugate of PGF2α covalently linked to bovine serum albumin (BSA) by reaction with carbodiimide reagent. A radioimmunoassay was developed using dextran-coated charcoal to separate the free from antibody-bound PGF2α-3H. The sensitivity of the method was about 100
William A. Brock, Burton V. Caldwell, Leon Speroff, Sumner Burstein   +3 more
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Prostaglandin F and E levels during endotoxin-induced pulmonary hypertension in calves.

American Journal of Physiology, 1975
Prostaglandin F and E (PGF and PGE) concentrations in sequential blood samples obtained simultaneously from the pulmonary artery (PA) and pulmonary vein (PV) during endotoxin-induced pulmonary hypertension in calves were determined by radioimmunoassay ...
F. Anderson, T. Tsagaris, W. Jubiz, H. Kuida   +3 more
semanticscholar   +1 more source

On agents favoring prostaglandin f formation during biosynthesis.

Biochemistry, 1975
The microsomal fraction of bovine vesicular gland catalyzed the conversion of eicosapolyenoic acids exclusively to prostaglandin E in the presence of reduced glutathione, while hydrox fatty acids, prostaglandins D and F, decreased to a negligible level ...
J. Chan, M. Nagasawa, C. Takeguchi, C. Sih   +3 more
semanticscholar   +1 more source

Onset of oestrus and fertility of dairy heifers and suckled beef cows treated with prostaglandin F 2α

, 1975
Suckled cows (average 70 days post partum) and British Friesian dairy heifers (13 to 24 months of age) were injected intramuscularly with prostaglandin F 2α (PGF 2α ) on two occasions 12 days apart, to synchronize oestrus and ovulation.
H. Hafs, J. Manns, B. Drew
semanticscholar   +1 more source

Synthetic Modification of Prostaglandin F2α Indicates Different Structural Determinants for Binding to the Prostaglandin F Receptor Versus the Prostaglandin Transporter [PDF]

Molecular Pharmacology, 2000
Several principles governing the binding of E series prostaglandins to EP receptors have emerged in recent years. The C-1 carboxyl group binds electrostatically to a conserved arginine residue in the seventh transmembrane segment of the receptor. Prostaglandin E analogs involving bioisosteric replacements of the carboxyl group, such as acylsulfonamide,
June Chen, Robert M. Burk, Shigekazu Itoh, Steven W. Andrews, Karen M. Kedzie, Daniel W. Gil, Victor L. Schuster, David F. Woodward   +7 more
openaire   +2 more sources

Progesterone production by dispersed cells from human corpus luteum: stimulation by gonadotrophins and prostaglandin F 2 alpha; lack of response to adrenaline and isoprenaline.

Journal of Endocrinology, 1980
Progesterone production was assessed following short-term incubations of luteal cell suspensions prepared from tissue samples of human corpora lutea obtained at specific times throughout the luteal phase of the menstrual cycle.
M. C. Richardson, G. Masson
semanticscholar   +1 more source

Roles of Prostaglandin F Synthase

2001
Prostaglandin (PG) F2 is widely distributed in various organs, and exhibits various biological actions such as smooth muscle contraction of uterus [1], bronchus [2], and trachea [3], the initiation of parturition [4], and pain transmission [5]. PGF synthase (PGFS) is a dual function enzyme, which catalyzes the reduction of not only PGD2 but also PGH2 ...
Motonari Fukui, Toshiko Suzuki-Yamamoto, Mikio Nishizawa, Seiji Ito, Kazunori Ishimura, Kikuko Watanabe   +5 more
openaire   +2 more sources

Parturition in goats: studies on the interactions between the foetus, placenta, prostaglandin F and progesterone before parturition, at term or at parturition induced prematurely by corticotrophin infusion of the foetus.

Journal of Endocrinology, 1977
Relationships between foetal corticosteroid concentrations, utero-ovarian prostaglandin F (PGF) and maternal peripheral progesterone have been examined in detail in goats shortly before spontaneous parturition at term.
W. B. Currie, G. Thorburn
semanticscholar   +1 more source

Intramyometrial prostaglandin F2α

American Journal of Obstetrics and Gynecology, 1972
Abstract Hypertonic saline and prostaglandin F 2α were injected into pregnant monkey myometrium. Small volumes of 20 per cent saline produced full-thickness myometrial necrosis with hemorrhage and thrombosis. PGF 2α in amounts sufficient to induce uterine contractions caused no histologic change.
Theodore M. King, Anne Colston Wentz
openaire   +3 more sources

Parturition in the goat: plasma concentrations of prostaglandin F and steroid hormones and uterine activity during late pregnancy and parturition.

Journal of Endocrinology, 1976
Changes in plasma concentrations of hormones and uterine activity associated with spontaneous parturition in the goat were examined. No change in oestradiol-17beta concentration was detected during the experimental period, but oestradiol-17alpha ...
I. Umo, R. Fitzpatrick, W. Ward
semanticscholar   +1 more source