Results 131 to 140 of about 16,987,595 (305)

Smaller is better: nanobodies meet NMR

FEBS Letters, EarlyView.
Nanobodies are single‐domain antigen‐binding fragments derived from camelid heavy chain antibodies. Their small size, high stability, and exceptional specificity make nanobodies uniquely useful probes for NMR studies of protein dynamics, transient conformational states, and protein–protein interactions.
Oleg Y. Dmitriev
wiley   +1 more source

Neutralization of the PTX3/CD44 axis suppresses TGF‐β1 signaling in kidney fibrosis

FEBS Letters, EarlyView.
This study investigates how the PTX3/CD44 axis promotes kidney fibrosis by activating TGF‐β1 signaling. We demonstrate that the neutralizing antibody WHC‐001 effectively inhibits this pathway, suppressing myofibroblast activation and partial epithelial–mesenchymal transition (EMT).
Chao‐Chun Cheng, Shen‐Shin Chang, Feng‐Wei Chen, Ying‐Chieh Su, Thanh Phuong Nguyen, Yu‐Wei Hsiao, Jhih‐Ying Chi, Hsin‐Yin Liang, Ju‐Ming Wang   +8 more
wiley   +1 more source

Inhibition of dipeptidyl peptidase 7 impairs lysophagy in LLOMe‐treated HepG2 cells

FEBS Letters, EarlyView.
DPP7 inhibition delays ubiquitin‐dependent lysophagy by attenuating ubiquitin accumulation and the recruitment of SQSTM1 and LC3 to damaged lysosomes, resulting in delayed clearance of damaged lysosomes and impaired restoration of lysosomal homeostasis. Created using FigureLabs (Certificate ID: FL‐PUB‐20260922‐Z99XNX).
Na Yeon Park, Inho Kwon, Yong Hwan Kim, Ji‐Eun Bae, Joon Bum Kim, Seong Hyun Kim, Sungho Maeng, Doo Sin Jo, Dong‐Hyung Cho   +8 more
wiley   +1 more source

Artificial intelligence-driven computational methods for antibody design and optimization

mAbs
Antibodies play a crucial role in our immune system. Their ability to bind to and neutralize pathogens opens opportunities to develop antibodies for therapeutic and diagnostic use.
Luiz Felipe Vecchietti, Bryan Nathanael Wijaya, Azamat Armanuly, Begench Hangeldiyev, Hyunkyu Jung, Sooyeon Lee, Meeyoung Cha, Ho Min Kim   +7 more
doaj   +1 more source

DNA ligase LigE increases bacterial transformation with damaged extracellular DNA

FEBS Letters, EarlyView.
The DNA ligase LigE from the naturally competent Gram‐negative bacterium Neisseria gonorrhoaea increases transformation with cut extracellular DNA. This process is ATP‐dependent and relies on the catalytic activity of LigE; it also requires that LigE contains an intact periplasmic localisation sequence, indicating that ligE functions in the periplasm ...
Jolyn Pan, Avi Singh, Shrawani Weralupe Gamladdalage, Joanna Hicks, Adele Williamson   +4 more
wiley   +1 more source

AbDesign: database of point mutants of antibodies with associated structures reveals poor generalization of binding predictions from machine learning models

mAbs
Antibodies are naturally evolved molecular recognition scaffolds that can bind a variety of surfaces. Their designability is crucial to the development of biologics, with computational methods holding promise in accelerating the delivery of medicines to ...
Bartosz Janusz, Dawid Chomicz, Samuel Demharter, Marloes Arts, Jurrian de Kanter, Yano Wilke, Helena Britze, Sonia Wrobel, Tomasz Gawłowski, Pawel Dudzic, Kärt Ukkivi, Lauri Peil, Roberto Spreafico, Konrad Krawczyk   +13 more
doaj   +1 more source

Going viral − Virus‐targeting nanobodies for research, diagnostics and therapy

FEBS Letters, EarlyView.
The increasing prevalence of viral diseases represents a major threat for global human health. Nanobodies, small binding fragments derived from camelid heavy‐chain antibodies, offer versatile tools for viral research, diagnostics, and antiviral therapy.
Domenic Ebert, Ulrich Rothbauer
wiley   +1 more source

Directed evolution of chemotaxis inhibitory protein of Staphylococcus aureus generates biologically functional variants with reduced interaction with human antibodies

, 2009
Chemotaxis inhibitory protein of Staphylococcus aureus (CHIPS) is a protein that binds and blocks the C5a receptor (C5aR) and formylated peptide receptor, thereby inhibiting the immune cell recruitment associated with inflammation.
Erika Gustafsson, Christina Furebring, Kok P.M. van Kessel, van Strijp, Jos A.G., Karin Barchan, Haas, Pieter-Jan, Forsberg, Cecilia, Ljung, Lill, Haraldsson, Karin, Walse, Björn, Björn Walse, Rosén, Anna, Gustafsson, Erika,, Anna Rosén, Karin Bryder, Bryder, Karin, Barchan, Karin, Lindman, Stina, Stina Lindman, Gustafsson, Erika, Pieter-Jan Haas, Cecilia Forsberg, Jos A.G. van Strijp, Lindman, Stina,, Lill Ljung, Furebring, Christina, Karin Haraldsson, Lund University., Kessel, Kok P.M.   +28 more
core   +1 more source

Soft motions encoded by the fold and mediated by conserved hinge sites drive V‐ATPase allosteric machinery and autoinhibition

FEBS Letters, EarlyView.
Vacuolar H+‐ATPase (V‐ATPase) is a multisubunit rotary proton pump that acidifies intracellular compartments. Using elastic network models, we identified conserved hinge residues that govern its large‐scale functional motions. We show that intrinsically encoded ‘soft modes’ drive critical functional transitions, including rotary catalysis and ...
Diksha Parwana, Xiaowei Bogetti, Stuti Sharma, Ivet Bahar   +3 more
wiley   +1 more source

What is sought from graphic designers? A first thematic analysis of job offers for graphic design positions in the United Kingdom

, 2016
An empirically grounded understanding about which knowledge and skills that are sought from designers is missing for a number of professional subfields of design. This gap in research challenges i) design educators in planning their educational offerings
Person, Oscar, Nicoletti Dziobczenski, Paulo, Dziobczenski, Paulo Roberto Nicoletti, Paulo Roberto Nicoletti Dziobczenski, Oscar Person   +4 more
core   +1 more source