Results 171 to 180 of about 1,706,932 (293)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Pantanal virophage: a new virophage species associated with a moumouvirus and a transpoviron, expanding the genus <i>Sputnikvirus</i>. [PDF]

J Virol
de Azevedo BL, Arias NEC, Machado TB, Pessoa Araújo Júnior J, Alves AKdNN, Alcantara LCJ, Aylward FO, Rosa LH, Rodrigues RAL, Abrahão JS.   +9 more
europepmc   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

High-Affinity Six-Letter DNA XenoAptamer Generation by Genetic Alphabet Expansion. [PDF]

J Am Chem Soc
Matsunaga KI, Tan HP, Low JL, Kimoto M, Hirao I.   +4 more
europepmc   +1 more source

Differential regulation of ZFAS1 splice variants by endoplasmic reticulum stress in hepatocyte cell lines

FEBS Open Bio, EarlyView.
ZFAS1 is a lncRNA promoting cell proliferation and migration, exhibiting high expression in various cancers. It is conserved, widely expressed, and produces multiple splice variants with unclear roles. We identified several splice variants in hepatocyte models, and found that inhibiting or suppressing regulators of the unfolded protein response (PERK ...
Sébastien Soubeyrand, Paulina Lau, Ruth McPherson   +2 more
wiley   +1 more source

The Semantics and Mechanisms of Enhancers and Promoters: "What Is True for <i>E. coli</i> Is True for the Elephant, Only More So". [PDF]

Annu Rev Biochem
Struhl K.
europepmc   +1 more source

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

FEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source

Microbial communities in filth flies collected from dairy and poultry farms for supplemental animal feed. [PDF]

J Econ Entomol
Olmstead M, Van Nest K, Swistek S, Cohnstaedt LW, Oppert B, Shults P.   +5 more
europepmc   +1 more source

BMI‐1 modulation and trafficking during M phase in diffuse intrinsic pontine glioma

FEBS Open Bio, EarlyView.
The schematic illustrates BMI‐1 phosphorylation during M phase, which triggers its translocation from the nucleus to the cytoplasm. In cycling cells, BMI‐1 functions within the PRC1 complex to mediate H2A K119 monoubiquitination. Following PTC596‐induced M phase arrest, phosphorylated BMI‐1 dissociates from PRC1 and is exported to the cytoplasm via its
Banlanjo Umaru, Deepak Kumar Mishra, Shiva Senthil Kumar, Hao‐Han Pang, Brendan Devine, Komal Khan, Rachid Drissi   +6 more
wiley   +1 more source

Operating CRISPR/Cas12a in a complex nucleic acid sequence background. [PDF]

Nucleic Acids Res
Hellmer H, Mayer T, Bauersachs L, Simmel FC.   +3 more
europepmc   +1 more source