Results 331 to 340 of about 8,970,337 (403)
FEBS Open Bio, EarlyView.We introduce an immunohistochemistry method to measure autophagy flux, highlighting the active degradation and recycling of cellular waste. This cost‐effective approach uses tissue samples to track key markers like LC3 and SQSTM1, revealing how cells maintain health or respond to diseases such as cancer. It bridges the gap between research and clinical
Shahla Shojaei +6 more
wiley +1 more source
Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays. [PDF]
J Vis Exp, 2018 Asher A, Ganesan I, Klasek L, Theg SM.
europepmc +1 more source
FEBS Open Bio, EarlyView.Heterologous expression of membrane transporters in cultured cells is essential for functional characterization, but is sometimes limited by low activity. Our study compares the HDAC inhibitors butyrate, VPA and SAHA to enhance transport activity. We propose to replace butyrate by SAHA: it is equally effective, devoid of repulsive odor, costs less, and
Svenja Flögel +4 more
wiley +1 more source
Gating Protein Transport in Solid State Nanopores by Single Molecule Recognition. [PDF]
ACS Cent Sci, 2018 Emilsson G +6 more
europepmc +1 more source
The cytoplasmic dynein transport machinery and its many cargoes
Nature reviews. Molecular cell biology, 2018 Samara L. Reck-Peterson +3 more
semanticscholar +1 more source
FEBS Open Bio, EarlyView.Measuring AMP‐activated protein kinase (AMPK) activity in vitro is crucial for testing AMPK activators or inhibitors with therapeutic potential. Here, we report an enzyme‐linked immunosorbent assay (ELISA)‐based AMPK activity assay with simple steps and high sensitivity, which offers a simple, robust, and cost‐effective alternative to traditional ...
Trezze P. Nguyen, Shangze Lyu, Yang Liu
wiley +1 more source
A gene-rich mitochondrion with a unique ancestral protein transport system
Moreira D, Blaz J, Kim E, Eme L.
europepmc +1 more source
SDS-assisted protein transport through solid-state nanopores. [PDF]
Nanoscale, 2017 Restrepo-Pérez L +4 more
europepmc +1 more source
The C9orf72 repeat expansion disrupts nucleocytoplasmic transport
Nature, 2015 Ke Zhang +20 more
semanticscholar +1 more source
FEBS Open Bio, EarlyView.The tumor microenvironment is a dynamic, multifaceted complex system of interdependent cellular, biochemical, and biophysical components. Three‐dimensional in vitro models of the tumor microenvironment enable a better understanding of these interactions and their impact on cancer progression and therapeutic resistance.
Salma T. Rafik +3 more
wiley +1 more source