Results 271 to 280 of about 7,056,691 (346)

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Sources, impacts and mechanisms of non-pharmacological artefacts in processed electroencephalography monitoring during anaesthesia and intensive care: a scoping review protocol. [PDF]

BMJ Open
Beg A, Kim CN, Tung A.
europepmc   +1 more source

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

FEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita   +3 more
wiley   +1 more source

Tailored charging protocol for densified lithium deposition and stable initially anode-free lithium metal pouch cells. [PDF]

Nat Commun
Liu Y, Yin X, Guo H, Wang S, Li B, Chen G.   +5 more
europepmc   +1 more source

Navigating new norms: a systematic review of factors for the development of effective digital tools in higher education

FEBS Open Bio, EarlyView.
What factors make for an effective digital learning tool in Higher Education? This systematic review identifies elements of a digital tool that published examples reveal to be features of an engaging and impactful digital tool. A systematic literature search yielded 25 research papers for analysis.
Akmal Arzeman, Jessica Haines, Connie Pritchard, Stephen Rutherford, Nigel Francis   +4 more
wiley   +1 more source

Outcome measures used in the conservative management of people with non-specific thoracic spine pain (NTSP): a scoping review protocol. [PDF]

BMJ Open
Kovanur Sampath K, Fleischmann M, Vaughan B.   +2 more
europepmc   +1 more source

Hands‐on protocol for preparing water‐soluble fractions from agri‐food samples for NMR‐based metabolomics analysis

FEBS Open Bio, EarlyView.
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso, Jaime Hiniesta‐Valero, Alejandra Guerra‐Castellano, Miguel A. De la Rosa, Irene Díaz‐Moreno   +4 more
wiley   +1 more source

Harnessing non-equilibrium forces to optimize work extraction. [PDF]

Nat Commun
Olsen KS, Goerlich R, Roichman Y, Löwen H.   +3 more
europepmc   +1 more source

Experimentally achieving minimal dissipation via thermodynamically optimal transport. [PDF]

Nat Commun
Oikawa S, Nakayama Y, Ito S, Sagawa T, Toyabe S.   +4 more
europepmc   +1 more source

Telemedicine-based individualised aerobic exercise training in Chinese adults with inactive or mildly active inflammatory bowel disease: study protocol for a single-centre, semi-crossover randomised controlled trial. [PDF]

BMJ Open
Zhou Y, Liu H, Qian X, Zhang X, Xu F.
europepmc   +1 more source