Results 161 to 170 of about 7,610 (197)
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The Proton-Translocating ATPase of Escherichia Coli
Annual Review of Biophysics and Biophysical Chemistry, 1990The purpose of this review is to provide an up-to-date summary of E. coli proton-translocating F1F0ATPase. From work on this enzyme, new insights have been gained in the areas of bacterial physiology and energy metabolism, mechanism of enzyme action, mechanism of ion transport through membranes, structure of membrane proteins, mechanism of energy ...
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Paracoccus denitrificans proton-translocating ATPase: Kinetics of oxidative phosphorylation
Biochemistry (Moscow), 2010The initial rates of ATP synthesis catalyzed by tightly coupled Paracoccus denitrificans plasma membrane were measured. The reaction rate was hyperbolically dependent on the substrates, ADP and inorganic phosphate (P(i)). Apparent K(m) values for ADP and P(i) were 7-11 and 60-120 µM, respectively, at saturating concentration of the second substrate (pH
K A, Kegyarikova +2 more
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The proton-translocating ATPase of Candida tropicalis plasma membrane
Biochimie, 1982Proton translocation activity of Candida tropicalis plasma membrane ATPase has been demonstrated using a fluorescent delta pH probe (ACMA) and by direct pH measurements. Modifications in fluorescence intensity and H+ transport are highly specific for Mg2+ and ATP, and are sensitive to the well-known inhibitors of the plasma membrane ATPase, vanadate ...
F, Blasco, X, Gidrol
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Control of proton translocation induced by ATPase activity in chloroplasts
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 19751. Proton uptake was induced by ATP in the dark following light triggering of ATPase activity in chloroplasts. The accumulated protons were released when ATPase activity was inhibited by the energy transfer inhibitor DIO-9. 2. Approximately two protons were taken up for each ATP hydrolyzed at pH 8.
C, Carmeli, Y, Lifshitz, A, Gepshtein
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Proton translocating ATPase mediated fungicidal activity of eugenol and thymol
Fitoterapia, 2010Eugenol (1) and thymol (2) exhibit excellent fungicidal activity against pathogenic yeasts, including isolates resistant to azoles. The rapid irreversible action of compound-1 and compound 2 on fungal cells suggested a membrane-located target for their action. We investigated their effect on H(+)-ATPase mediated H(+)-pumping by various Candida species.
Aijaz, Ahmad +4 more
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Proton-Translocating ATPase (F1F0) of Escherichia coli
1982Studies on proton-translocating ATPase (F1F0) from Escherichia coli started much later than those on F1F0 from eukaryotic organelles such as mitochondria and chloroplasts. The F1-ATPase of E. coli was purified after solubilization from cytoplasmic membranes and was shown to have essentially the same function and five-subunit structure (α, β, γ, δ ...
Masamitsu Futai, Hiroshi Kanazawa
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Proton translocation in corn coleoptiles: ATPase or redox chain?
Planta, 1985The O2 dependence of net H(+) efflux of maize coleoptiles has been investigated. Below 100 μM O2, H(+) efflux in young (1 cm long) coleoptiles is markedly decreased while old (7 cm long) coleoptiles show a decline only at 10 μM O2. Old coleoptiles show the same decrease in net H(+) efflux as young ones if treated with fusicoccin.
M, Böttger, M, Bigdon, H J, Soll
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Advances in Biophysics, 1987
Recent results on ATPase, mainly from E. coli, obtained by biochemical and molecular biological approaches are reviewed, with special emphasis on results obtained in this laboratory. The advantages of using E. coli in studies of this important enzyme in oxidative phosphorylation are indicated: variant enzymes with specific amino acid replacements can ...
M, Futai, T, Noumi, M, Maeda
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Recent results on ATPase, mainly from E. coli, obtained by biochemical and molecular biological approaches are reviewed, with special emphasis on results obtained in this laboratory. The advantages of using E. coli in studies of this important enzyme in oxidative phosphorylation are indicated: variant enzymes with specific amino acid replacements can ...
M, Futai, T, Noumi, M, Maeda
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Proton-translocating ATPase from bovine kidney medulla: partial purification and reconstitution
American Journal of Physiology-Renal Physiology, 1988The proton-translocating ATPase that is responsible both for urinary and vacuolar acidification was partially purified from bovine kidney medulla microsomes. ATPase activity was purified to a maximum specific activity of 1.7 mumol.min-1.mg prot-1 and was inhibited completely by N-ethylmaleimide. The relative molecular weight (Mr) of the intact protein
S, Gluck, J, Caldwell
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Effect of diamide on proton translocation by the mitochondrial H+-ATPase
Biochemical and Biophysical Research Communications, 1985Treatment of sonic submitochondrial particles with the bifunctional thiol reagent, diamide, results in an enhancement of proton conductivity and ATPase activity, which is reversed by the reducing agent dithiothreitol, is suppressed by Fo inhibitors like oligomycin and is absent in particles that are deprived of peripheral Fo polypeptides. The effect of
F, Zanotti +4 more
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