Results 261 to 270 of about 40,345 (282)

In vivo transfection of bovine leukemia provirus into sheep

Virology, 1992
Bovine leukemia virus is horizontally transmitted mainly through infected cells by direct blood transfer. In this report, a cloned bovine leukemia virus (BLV) provirus was examined for its infectivity by direct inoculation into sheep. One hundred micrograms of plasmid DNA containing a complete provirus was mixed with a cationic liposome solution and ...
Willems, Lucas, Portetelle, Daniel, Chen, Guangling, Burny, Arsène, Kettmann, Richard, Kerkhofs, Pierre, Mammerickx, Marc   +6 more
openaire   +3 more sources

Retroviruses in foreign species and the problem of provirus silencing

Gene, 2000
Retroviruses are known to integrate in the host cell genome as proviruses, and therefore they are prone to cell-mediated control at the transcriptional and posttranscriptional levels. This plays an important role especially after retrovirus heterotransmission to foreign species, but also to differentiated cells. In addition to host cell-mediated blocks
Z. Vernerová, Jiří Hejnar, Jan Svoboda, Josef Geryk, Daniel Elleder   +4 more
openaire   +3 more sources

Excision of an integrated provirus by the action of FLP recombinase

In Vitro Cellular & Developmental Biology - Animal, 1997
Retroviral vectors can be used to insert a single, intact copy of a transgene into a chromosome. If the duplication of the LTR (long-terminal repeat) that naturally occurs during reverse transcription of the retroviral genome is exploited to introduce two equally oriented FLP recognition target (FRT) sites, a substrate for FLP recombinase is created. A
Dirk Schübeler, Christian Mielke, Jürgen Bode   +2 more
openaire   +3 more sources

Unusual DNA structures at the integration site of an HIV provirus

Biochemical and Biophysical Research Communications, 1989
Supercoiled pHXBc2 DNA (containing the genome of the human immunodeficiency virus type 1 and human sequences) migrated more slowly than linear DNA in native and ethidium bromide agarose gel electrophoresis at 4.5 volts/cm, suggesting the presence of unusual DNA structures. S1 nuclease analysis of pHXBc2 revealed two S1 hypersensitive sites.
Karen E. Schneider, Ruth M. Ruprecht, Jessica C. Hall, Gregory C. Lukaszewicz, Miguel A. Gama Sosa   +4 more
openaire   +3 more sources

Chromosome integration domain for bovine leukemia provirus in tumors

Journal of Virology, 1983
The 3'-end host-virus junction fragments from two bovine leukemia virus (BLV)-induced lymphoid tumors (tumors 15-4 and 1351), each containing a single provirus, were used as probes to detect large restriction fragments flanking these proviruses. The DNAs from 28 other independent BLV-induced tumors were checked by Southern analysis of their restriction
D. Couez, Richard Kettmann, Rodolphe Palm, Jean-Jacques Claustriaux, Arsène Burny, Jacqueline Deschamps   +5 more
openaire   +3 more sources

Demonstration of a DNA Provirus in the Lytic Growth of Visna Virus

Nature New Biology, 1973
SEVERAL lines of evidence link the RNA viruses responsible for slow diseases in Icelandic sheep with RNA tumour viruses. Visna virus, the prototype of the RNA slow virus group, resembles RNA tumour viruses in its morphology, in maturating by budding from the surfaces of infected cells1 and in having a high molecular weight RNA sedimenting at 60–70S2–4 ...
Ashley T. Haase, Harold E. Varmus
openaire   +3 more sources

Synthesis, Integration, and Transcription of the Retroviral Provirus

1983
The RNA genome of a retrovirus is copied into DNA by the viral enzyme, RNA-dependent DNA polymerase, and the DNA copy of the retrovirus genome is inserted into one of the host’s chromosomes. This DNA copy, called a provirus, is transcribed into RNA by host enzymes, giving rise to both viral genomic RNA and viral messenger RNA.
openaire   +3 more sources

Sequence of retrovirus provirus resembles that of bacterial transposable elements

Nature, 1980
The nucleotide sequences of the terminal regions of an infectious integrated retrovirus cloned in the modified lambda phage cloning vector Charon 4A have been elucidated. There is a 569-base pair direct repeat at both ends of the viral DNA. The cell-virus junctions at each end consist of a 5-base pair direct repeat of cell DNA next to a 3-base pair ...
Kunitada Shimotohno, Howard M. Temin, Satoshi Mizutani   +2 more
openaire   +3 more sources

Correlation between HIV provirus burden and in utero transmission

AIDS, 1993
No predictive parameters of in utero or perinatal vertical transmission of HIV to newborns are known at present. Vertical transmission may be related to several biological parameters of maternal HIV infection: (1) immunological parameters (neutralizing antibodies); (2) the concentration of viral particles and/or infected cells; and (3) the selection of
C. Courpotin, Marie-Caroline Meyohas, Remy Narwa, Hervé F, Dominique Dormont, Françoise Parnet Mathieu, F. D. Boussin, Catherine Dollfus, Dominique Marcé, Pierre Roques   +9 more
openaire   +2 more sources

A thaumarchaeal provirus testifies for an ancient association of tailed viruses with archaea.

Biochemical Society Transactions, 2011
Archaeal viruses, or archaeoviruses, display a wide range of virion morphotypes. Whereas the majority of those morphotypes are unique to archaeal viruses, some are more widely distributed across different cellular domains.
M. Krupovic, A. Spang, S. Gribaldo, P. Forterre, C. Schleper   +4 more
semanticscholar   +1 more source