Results 11 to 20 of about 13,226 (233)

On the generation of the MSD-Ѱ class of defective HIV proviruses [PDF]

Retrovirology, 2019
Antiretroviral therapy (ART) can effectively suppress ongoing HIV replication and block disease progression, but the infection is never cured due to the persistence of a small pool of latently infected cells hosting integrated replication-competent HIV ...
Atze T. Das, Alexander O. Pasternak, Ben Berkhout   +2 more
doaj   +5 more sources

A systematic analysis of marine lysogens and proviruses

Nature Communications, 2023
Viruses are ubiquitous in the oceans, exhibiting high abundance and diversity. Here, we systematically analyze existing genomic sequences of marine prokaryotes to compile a Marine Prokaryotic Genome Dataset (MPGD, consisting of over 12,000 bacterial and ...
Yi Yi, Shunzhang Liu, Yali Hao, Qingyang Sun, Xinjuan Lei, Yecheng Wang, Jiahua Wang, Mujie Zhang, Shan Tang, Qingxue Tang, Yue Zhang, Xipeng Liu, Yinzhao Wang, Xi Xiao, Huahua Jian   +14 more
semanticscholar   +4 more sources

Defective HIV-1 proviruses produce viral proteins [PDF]

Proceedings of the National Academy of Sciences, 2020
Significance In HIV-infected patients on combination antiretroviral therapy (cART), greater than 95% of proviruses in the peripheral blood are “defective.” Historically, these defective proviruses have been thought to be dead-end products with no real ...
H. Imamichi, Mindy Smith, J. Adelsberger, Taisuke Izumi, F. Scrimieri, Brad T. Sherman, Catherine A. Rehm, T. Imamichi, A. Pau, M. Catálfamo, A. Fauci, H. Lane   +11 more
semanticscholar   +3 more sources

Defective proviruses rapidly accumulate during acute HIV-1 infection [PDF]

Nature Medicine, 2016
Although antiretroviral therapy (ART) suppresses viral replication to clinically undetectable levels, human immunodeficiency virus type 1 (HIV-1) persists in CD4+ T cells in a latent form that is not targeted by the immune system or by ART.
Katherine M. Bruner, Alexandra J Murray, Ross A. Pollack, M. Soliman, S. Laskey, A. Capoferri, J. Lai, M. Strain, Steven M. Lada, R. Hoh, Y. Ho, D. Richman, S. Deeks, J. Siliciano, R. Siliciano   +14 more
semanticscholar   +5 more sources

Identification of Genetically Intact HIV-1 Proviruses in Specific CD4+ T Cells from Effectively Treated Participants

Cell Reports, 2017
Latent replication-competent HIV-1 persists in individuals on long-term antiretroviral therapy (ART). We developed the Full-Length Individual Proviral Sequencing (FLIPS) assay to determine the distribution of latent replication-competent HIV-1 within ...
Bonnie Hiener, Bethany A. Horsburgh, John-Sebastian Eden, Kirston Barton, Timothy E. Schlub, Eunok Lee, Susanne von Stockenstrom, Lina Odevall, Jeffrey M. Milush, Teri Liegler, Elizabeth Sinclair, Rebecca Hoh, Eli A. Boritz, Daniel Douek, Rémi Fromentin, Nicolas Chomont, Steven G. Deeks, Frederick M. Hecht, Sarah Palmer   +18 more
doaj   +2 more sources

Structural rearrangements in the nucleus localize latent HIV proviruses to a perinucleolar compartment supportive of reactivation [PDF]

Proceedings of the National Academy of Sciences of the United States of America
Significance Persistent viral reservoirs in memory T cells harbor latent replication-competent proviruses that can rebound after interruption of antiretroviral therapy.
Fredrick Kizito, Kien Nguyen, Uri Mbonye, Meenakshi Shukla, Benjamin G. Luttge, Mary Ann Checkley, A. V. Agaponova, Konstantin Leskov, Jonathan Karn   +8 more
openalex   +2 more sources

Proviruses with Long-Term Stable Expression Accumulate in Transcriptionally Active Chromatin Close to the Gene Regulatory Elements: Comparison of ASLV-, HIV- and MLV-Derived Vectors

Viruses, 2018
Individual groups of retroviruses and retroviral vectors differ in their integration site preference and interaction with the host genome. Hence, immediately after infection genome-wide distribution of integrated proviruses is non-random.
Dalibor Miklík, Filip Šenigl, Jiří Hejnar   +2 more
doaj   +2 more sources

Genetic Diversity, Compartmentalization, and Age of HIV Proviruses Persisting in CD4 ⁺ T Cell Subsets during Long-Term Combination Antiretroviral Therapy

Journal of Virology, 2019
The main barrier to HIV cure is the ability of a genetically diverse pool of proviruses, integrated into the genomes of infected CD4+ T cells, to persist despite long-term suppressive combination antiretroviral therapy (cART).
Bradley R. Jones, Rachel L. Miller, Natalie N. Kinloch, Olivia Tsai, Hawley Rigsby, Hanwei Sudderuddin, Aniqa Shahid, Bruce Ganase, Chanson J. Brumme, Marianne Harris, Art F. Y. Poon, Mark A. Brockman, Rémi Fromentin, Nicolas Chomont, Jeffrey B. Joy, Zabrina L. Brumme   +15 more
openalex   +3 more sources

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing [PDF]

Journal of Visualized Experiments, 2018
The Full-Length Individual Proviral Sequencing (FLIPS) assay is an efficient and high-throughput method designed to amplify and sequence single, near full-length (intact and defective), HIV-1 proviruses.
Bonnie Hiener, John‐Sebastian Eden, Bethany A. Horsburgh, Sarah Jane Palmer   +3 more
openalex   +2 more sources

Within-Subtype HIV-1 Polymorphisms and Their Impacts on Intact Proviral DNA Assay (IPDA) for Viral Reservoir Quantification [PDF]

Viruses
The Intact Proviral DNA Assay (IPDA) is widely used to quantify genome-intact HIV proviruses in people living with HIV, but viral sequence diversity has been observed to cause assay failures due to primer/probe mismatches.
Mohith Reddy Arikatla, Jyoti S. Mathad, Kavidha Reddy, Nicole Reddy, Thumbi Ndung’u, Kathryn M. Dupnik, Guinevere Q. Lee   +6 more
doaj   +2 more sources