Results 41 to 50 of about 3,766 (164)

Chloroplast Genome Insights of Gleditsia japonica var. velutina and Evolutionary Implications for Variation and Phylogeny in Gleditsia

Ecology and Evolution, Volume 16, Issue 6, June 2026.
This study presents the first comprehensive chloroplast genome analysis of the critically endangered G. japonica var. velutina, providing essential insights into its evolutionary divergence within the genus Gleditsia and offering a critical genomic foundation for its conservation.
Shiyu Hu, Naiwei Li, Xingjian Liu, Baocheng Wu, Yueqi Sun, Mimi Li   +5 more
wiley   +1 more source

Validating Oxford Nanopore Technologies Amplicon Sequencing as an Alternative to Sanger Sequencing for Generating Plant Molecular Markers

New Zealand Journal of Botany, Volume 64, Issue 2, June 2026.
Molecular phylogenetic markers used to be ubiquitously obtained by Sanger sequencing, while recently, next generation sequencing (NGS) approaches have become popular alternatives. In this study, a pipeline utilizing Oxford Nanopore Technologies (ONT) amplicon sequencing (amplicon‐seq) was examined as an alternative to Sanger sequencing, with nine loci [
Kanae Nishii, Michelle L. Hart, Laura L. Forrest, M. K. Akhil, Santhosh Nampy, Subhani Ranasinghe, Aaron Jeffries, Michael Möller   +7 more
wiley   +1 more source

Leveraging target enrichment and genome skimming (Hyb‐Seq) of herbarium collections to unlock timber DNA barcoding

Applications in Plant Sciences, Volume 14, Issue 3, May-June 2026.
Abstract Premise DNA barcoding for timber species identification requires comprehensive reference datasets, informative DNA barcodes, and cost‐effective protocols. We developed a workflow leveraging Hyb‐Seq (target capture sequencing and genome skimming) to address these challenges, and we tested it on four genera from the mahogany family (Meliaceae ...
Sidonie Bellot, Dyana Ndiade Bourobou, Camila Quintero‐Berns, Laszlo Csiba, Peter Gasson, Barrett McBride, Bhely Angoboy Ilondea, Gaël U. D. Bouka, Emmanuel Ebanyenle, Cynel Gwenael Moundounga, Martin Ricker, Philomena Yarwoah, Guy Herman Zanguim Tchoutezou, Janvier Lisingo, Victor Deklerck   +14 more
wiley   +1 more source

Species identification of Rhododendron (Ericaceae) using the chloroplast deoxyribonucleic acid PsbA-trnH genetic marker

Pharmacognosy Magazine, 2012
Rhododendron is a group of famous landscape plants with high medicinal value. However, there is no simple or universal manner to discriminate the various species of this group. Deoxyribonucleic acid (DNA) barcoding technique is a new biological tool that can accurately and objectively identify species by using short and standard DNA regions.To choose a
Liu, Yimei, Zhang, Lehua, Liu, Zhen, Luo, Kun, Chen, Shilin, Chen, Keli   +5 more
openaire   +3 more sources

3RAD‐Guided SNP Discovery for Species Identification and Conservation of the Medicinal Southern African Tree Genus Greyia Hook. & Harv.

Ecology and Evolution, Volume 16, Issue 5, May 2026.
A two‐phase SNP discovery and assay‐development strategy was used to resolve species boundaries within the tree genus Greyia, as overlapping phenotypes and distributions limit accurate identification. From 47,726 genome‐wide SNPs generated by 3RAD sequencing, a diagnostic 23‐SNP Type panel was developed and validated, successfully distinguishing G ...
Iné Botha, Simo N. Maduna, Snorre B. Hagen, Namrita Lall, Dave K. Berger   +4 more
wiley   +1 more source

A two-locus global DNA barcode for land plants: the coding rbcL gene complements the non-coding trnH-psbA spacer region. [PDF]

PLoS ONE, 2007
BACKGROUND: A useful DNA barcode requires sufficient sequence variation to distinguish between species and ease of application across a broad range of taxa.
W John Kress, David L Erickson
doaj   +1 more source

IDENTIFICATION OF LENS CULTIVARS IN MARKET BY MOLECULAR TOOLS: DNA BARCODING AND SSRs

Trakya University Journal of Natural Sciences, 2023
Substitution of plant cultivars of high commercial value with a cheaper, lower quality one is a common fraud committed against consumers and producers. Since it is one of the most widely grown legumes, lentil (Lens culinaris Medik.) is suitable for such ...
Metin Burak Tatlıses, Semra Hasancebi
doaj   +1 more source

Integration of Genome‐Skimming Sequencing and Morphological Evidence Reveals Two New Endemic Species of Sinocrassula From Yunnan Province, China

Ecology and Evolution, Volume 16, Issue 5, May 2026.
Sinocrassula crassifolia. ABSTRACT Sinocrassula represents the medium‐sized Asian genus within Crassulaceae, exhibiting maximum species diversity in China. This study presented the first comprehensive phylogenetic analyses of Sinocrassula, utilizing both sanger data and next‐generation sequencing data.
Jing Zhao, Ling‐Nan Wei, Jie Zhou, Jian‐Rong Zhang, Xin‐Mao Zhou, Zhao‐Rong He, Chao Chen, Jia‐Guan Wang   +7 more
wiley   +1 more source

Identification of three kinds of Plumeria flowers by DNA barcoding and HPLC specific chromatogram

Journal of Pharmaceutical Analysis, 2018
DNA barcoding and HPLC specific chromatogram were used to identify three kinds of Plumeria flowers respectively. DNAs extracted from the three Plumeria species were amplified by PCR with universal primers, and the psbA-trnH region was selected.
Leilei Zhao, Xiaoxue Yu, Jie Shen, Xinjun Xu   +3 more
doaj   +1 more source

Complete Chloroplast Genomes of Ranunculus arvensis and Ranunculus laetus: Comparative Analysis and Phylogenetic Insights

Ecology and Evolution, Volume 16, Issue 5, May 2026.
Here, we de novo assembled the chloroplast genomes of Ranunculus arvensis and Ranunculus laetus, providing comprehensive comparative analysis with 21 congeners. The genomes exhibited conserved quadripartite structures with no major rearrangements, though lineage‐specific variations in atpH position were detected in two species.
Hui Li, Siyue Zhang, Abdullah, Sayed Afzal Shah, Yuhua Huang, Jingjing Jia, Liya Guo, Ying Cui, Jiahui Sun, Parviz Heidari, Xiaoxuan Tian   +10 more
wiley   +1 more source